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PAPANICOLAOU STAINING SOLUTIONS
(EA 36, EA 50, EA 65, and OG 6)

RECOMMENDED PROCEDURE:   Smears are alcohol-ether fixed. Smears are brought to distilled water through alcohols. Stain 2 - 3 minutes in Harris’ or Mayer’s Hematoxylin. Place in distilled water for 10 seconds. Rinse gently to prevent cells from being washed off (about 5 to 6 dips). Wash under running tap water for 6 minutes. Rinse in distilled water and transfer through graded alcohols; 10 seconds with dipping agitation in  50%, 70%, 80% and 95% alcohol. Stain in OG 6 solution for 1.5 minutes. Rinse smear dipping for 10 seconds in two individual changes of ethanol. If mucus is present, stain 1.5 minutes in    EA 36, EA 50 or EA 65. In each of 3 baths of 95% alcohol, rinse for 10 seconds. In each  of 2 baths of absolute ethyl alcohol, rinse for 10 seconds. Clear in 3 - 4 changes of xylene.

NOTE:  For smears other than cervical scrapings, e.g. bronchial aspirates, sputum, pleural and peritoneal fluids, EA 65 is substituted for EA 50 or EA 36.

RESULTS:  Following the complete Papanicolaou method of staining, results are as indicated: nuclei stain blue with clear, sharp detail; cytoplasm stain varying shades of pink, blue, yellow, green, and gray.

# 8000

Papanicolaou Staining Solution EA 36
1 Pack (Two 950 ml Btls.)

$ 102.00

# 8010

Papanicolaou Staining Solution EA 36
1 Gallon (3,800 ml)

125.00

# 8020

Papanicolaou Staining Solution EA 50
1 Pack (Two 950 ml Btls.)

102.00

# 8030

Papanicolaou Staining Solution EA 50
1 Gallon (3,800 ml)

125.00

# 8050

Papanicolaou Staining Solution EA 65
1 Pack (Two 950 ml Btls.)

110.00

# 8060

Papanicolaou Staining Solution EA 65
1 Gallon (3,800 ml)

125.00

# 8100

Papanicolaou Staining Solution 0G 6
1 Pack (Two 950 ml Btls.)

102.00

# 8110

Papanicolaou Staining Solution OG 6
1 Gallon (3,800 ml)

125.00

CONTENTS:   Eosin Y, Bismark Brown Y, Light Green SF, Yellowish, Orange G, Ethanol, Phosphotungstic Acid

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

SHORR-SINGLE DIFFERENTIAL STAIN

A stain for vaginal smears which provides a sharp differentiation between cornified and non-cornified elements.

RECOMMENDED PROCEDURE:   Fix wet specimen in equal parts of ethanol and ether. Stain for 1 minute. Carry through 70%, 95% and absolute alcohol - 10 dips in each solution. Clear in xylene and mount.

RESULTS:   Cornified elements stain a brilliant orange-red while the non-cornified elements take a green stain which is deeper in younger cells and paler in those more advanced. The staining is delicate and clearly reveals cytoplasmic and nuclear detail. WBC’s, RBC’s, bacteria and sperm are well-differentiated.

# 8120

Shorr-Single Differential Stain
1 Pack (Two 250 ml Btls.)

$  190.00

# 8130

Shorr-Single Differential Stain
1 Pack (Two 950 ml Btls.)

274.00

CONTENTS:   Ethanol, Biebrich Scarlet, Orange G, Fast Green FCF, Phosphotungstic Acid, Phosphomolybdic Acid, Glacial Acetic Acid

This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.

JENNER-GIEMSA STAIN KIT
(MAY-GRUNWALD GIEMSA METHOD)

Solution I - Jenner Stain (Stock)
Solution II - Giemsa Stain (Stock)

Designed for staining nuclei, cytoplasm and bacteria in tissue sections.

RECOMMENDED PROCEDURE:  Working Jenner Solution:  Mix 25 ml of Sol. I with 25 ml of distilled water.   Working Giemsa Solution:  Mix 50 drops of Sol. II with 50 ml. of distilled water.  NOTE:  This solution must be made fresh. Do not re-use. Prior to staining, specimen should be well-fixed, deparaffinized and cleared. Rinse in distilled water, 2 changes, then methanol, 2 changes at 3 minutes each. Stain in working Sol. I for 6 minutes. Stain in working Sol. II for 45 minutes. Handle each slide individually in this and subsequent steps. Differentiate in 1% glacial acetic acid, then check microscopically. Rinse in distilled water. Dehydrate in 95% ethanol, absolute ethanol and clear with xylene, 2 changes each.

RESULTS:  Nuclei-blue; cytoplasm-pink to rose; bacteria-blue.

# 8150

Jenner-Giemsa Stain Kit
1 Kit (100 ml Ea. Sol.)

$ 190.00

# 8151

Jenner Stain (Stock)
1 Btl. (100 ml)

100.00

# 8152

Jenner Stain (Stock)
1 Pack (Two 100 ml Btls.)

171.00

# 8153

Giemsa Stain (Stock)
1 Btl. (100 ml)

100.00

# 8154

Giemsa Stain (Stock)
1 Pack (Two 100 ml Btls.)

171.00

CONTENTS:   Jenner Stain, Giemsa Stain, Methanol, Glycerin

These solutions are made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.

GILL-HEMATOXYLIN STAIN KIT

Gill-Hematoxylin, 0.2% (Modified), 0.4% or 0.6%
Solution II - Scott’s Tap Water Substitute
Solution III - Ethanol (95%)

An excellent nuclear stain employing a half-oxidized hematoxylin solution. (Gill, 1974) Gill Hematoxylin is a high contrast stain and a progressive hematoxylin which enchances the use of counterstains. To achieve desired stain characteristics in different specimens, the stain is available in 3 concentrations at 0.2% w/v, (Modified), 0.4% w/v, and 0.6% w/v. The modified solution at 0.2% may be preferred in cytology, however, by increasing the time (10 minutes) it can be used in histology. Gill-Hematoxylin at 0.4% is recommended for histology, however, a shorter stain time renders this solution suitable for cytologic use. For comparable results to those obtained with Harris’ Hematoxylin, Gill-Hematoxylin at 0.6% is recommended. An essential part of this stain sequence is the use of a blueing agent. We recommend Scott’s Tap Water Substitute which ensures a controlled pH and aids in cell retention on slides.

RECOMMENDED PROCEDURE:  Gill-Hematoxylin (Modified): Fix in Sol. III for 15 minutes. Water - 2 x (10 dips each). Gill-Hematoxylin (Modified) - 2 minutes. Water - 2 x (10 dips each). Sol. II - 1 minute. Water - 2 x (10 dips each). Sol. III - 2 x (10 dips each). Continue with preferred staining method.  Gill-Hematoxylin 0.4 % or 0.6 %: Xylene - 3 x  (2 minutes each). Absolute Ethanol - 3 x (30 seconds each). Water - 2 x (10 dips each). Gill Hematoxylin 0.4% or 0.6% - 3 minutes. Water - 2 x (10 dips each). Sol. II - 2 minutes. Water - 2 x (10 dips each). Continue with preferred staining method.

RESULTS:  Nuclear chromatin stains blue to blue-black; cytoplasm faintly tinted.

# 8400

Gill-Hematoxylin Stain Kit (Modified)
1 Kit (950 ml Ea. Sol.)

$   159.00

# 8401

Gill-Hematoxylin Stain Kit (0.4%)
1 Kit (950 ml Ea. Sol.)

164.00

# 8402

Gill-Hematoxylin Stain Kit (0.6%)
1 Kit (950 ml Ea. Sol.)

184.00

# 8411

Gill-Hematoxylin (Modified)
1 Btl. (950 ml)

100.00

# 8411-1

Gill-Hematoxylin (Modified)
1 Btl. (475 ml)

77.00

# 8412

Gill-Hematoxylin (Modified)
1 Kit (Two 950 ml Btls.)

165.00

# 8413 Gill-Hematoxylin (Modified)
1 Gallon (3,800 ml)

250.00

# 8421 Gill-Hematoxylin (0.4%)
1 Btl. (950 ml)

145.00

# 8422 Gill-Hematoxylin (0.4%)
1 Pack (Two 950 ml Btls.)
201.00
# 8423 Gill-Hematoxylin (0.4%)
1 Gallon (3,800 ml)
237.00
# 8431 Gill-Hematoxylin (0.6%)
1 Btl. (950ml)
146.00
# 8432 Gill-Hematoxylin (0.6%)
1 Pack (Two 950 ml Btls.)
214.00
# 8433 Gill-Hematoxylin (0.6%)
1 Gallon (3,800 ml)
284.00
# 8441 Sol. II - Scott’s Tap Water Substitute
1 Btl. (950 ml)
70.00
# 8442 Sol. II - Scott’s Tap Water Substitute
1 Pack (Two 950 ml Btls.)
99.00
# 8443 Sol. II - Scott’s Tap Water Substitute
1 Gallon (3,800 ml)
138.00
# 8451 Sol. III - Ethanol (95%)
1 Btl. (950 ml)
68.00
# 8452 Sol. III - Ethanol (95%)
1 Pack (Two 950 ml Btls.)
102.00
# 8453 Sol. III - Ethanol (95%)
1 Gallon (3,800 ml)
150.00

CONTENTS:   Ethylene Glycol, Hematoxylin, Sodium Iodate, Aluminum Sulfate, Citric Acid, Magnesium Sulfate, Sodium Bicarbonate, Ethanol

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

HARRIS’ ALUM HEMATOXYLIN STAIN
(MERCURY FREE)

A regressive stain used in demonstrating nuclear chromatin. Stain time is usually 4 - 8 minutes; however, nature of the tissue and depth of staining may require a time increase to 10 - 15 minutes.

RESULTS:  Nuclei stain brownish-black, cytoplasm stain pale pink.

# 8490

Harris’ Alum Hematoxylin Stain (Mercury Free)
1 Btl. (250 ml)

$   116.00

# 8491

Harris’ Alum Hematoxylin Stain (Mercury Free)
1 Btl. (950 ml)

145.00

# 8492

Harris’ Alum Hematoxylin Stain (Mercury Free)
1 Pack (Two 950 ml Btls.)

214.00

# 8493

Harris’ Alum Hematoxylin Stain (Mercury Free)
1 Gallon (3,800 ml)

273.00

CONTENTS:   Hematoxylin, Ethanol, Potassium Alum

This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.

HARRIS’ ALUM HEMATOXYLIN STAIN
(MERCURY FREE WITH GLACIAL ACETIC ACID)

A regressive stain used in demonstrating nuclear chromatin. Stain time is usually 4 - 8 minutes; however, nature of the tissue and depth of staining may require a time increase to 10 - 15 minutes.

RESULTS:  Nuclei stain brownish-black, cytoplasm stain pale pink.  Nuclei stain brownish-black, cytoplasm stain pale pink.

# 8494

Harris’ Alum Hematoxylin Stain  (Mercury Free with Glacial Acetic Acid)
1 Btl. (250 ml)

$  125.00

# 8495

Harris’ Alum Hematoxylin Stain (Mercury Free with Glacial Acetic Acid)
1 Btl. (950 ml)

153.00

# 8496

Harris’ Alum Hematoxylin Stain (Mercury Free with Glacial Acetic Acid)
1 Pack (Two 950 ml Btls.)

253.00

# 8497

Harris’ Alum Hematoxylin Stain (Mercury Free with Glacial Acetic Acid)
1 Gallon (3,800 ml)

324.00

CONTENTS:   Hematoxylin, Ethanol, Potassium Alum   Hematoxylin, Ethanol, Potassium Alum, Glacial Acetic Acid

This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.

HARRIS’ ALUM HEMATOXYLIN STAIN
(AVAILABLE WITH OR WITHOUT GLACIAL ACETIC ACID)

A regressive stain used in demonstrating nuclear chromatin. Stain time is usually 4 - 8 minutes; however, nature of the tissue and depth of staining may require a time increase to 10 - 15 minutes.

RESULTS:  Nuclei stain brownish-black, cyptoplasm stain pale pink.

# 8500

Harris’ Alum Hematoxylin Stain
1 Btl. (250 ml)

$    82.00

# 8501

Harris’ Alum Hematoxylin Stain
1 Btl. (950 ml)

112.00

# 8502

Harris’ Alum Hematoxylin Stain
1 Pack (Two 950 ml Btls.)

159.00

# 8503

Harris’ Alum Hematoxylin Stain
1 Gallon (3,800 ml)

211.00

# 8404

Harris’ Alum Hematoxylin Stain with Glacial Acetic Acid
1 Btl. (475 ml)

110.00

# 8510

Harris’ Alum Hematoxylin Stain with Glacial Acetic Acid
1 Pack (Two 950 ml Btls.)

212.00

# 8511

Harris’ Alum Hematoxylin Stain with Glacial Acetic Acid
1 Gallon (3,800 ml)

250.00

CONTENTS:   Hematoxylin, Potassium Alum, Mercuric Oxide (Red), Ethanol, Glacial Acetic Acid (where applicable)

This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.

MAYER’S ACID ALUM HEMATOXYLIN STAIN

Stain may be used either progressively or regressively. Excellent as a counterstain and for diagnostic quick sections. Recommended staining time is 4 - 8 minutes depending upon desired stain intensity.

# 8550

Mayer’s Acid Alum Hematoxylin Stain
1 Btl. (250 ml)

$ 100.00

# 8551

Mayer’s Acid Alum Hematoxylin Stain
1 Btl. (950 ml)

114.00

# 8552

Mayer’s Acid Alum Hematoxylin Stain
1 Pack (Two 950 ml Btls.)

189.00

# 8553

Mayer’s Acid Alum Hematoxylin Stain
1 Gallon (3,800 ml)

228.00

# 8555

Mayer’s Acid Alum Hematoxylin Stain
1 Pack (Two 250 ml Btls.)

143.00

CONTENTS:   Hematoxylin, Citric Acid, Sodium Iodate, Potassium Alum

This solution is made from certified dye.
FOR IN VITRO DIAGNOSTIC USE ONLY.

WEIGERT’S IRON HEMATOXYLIN STAIN KIT

Solution I - Weigert’s Hematoxylin
Solution II - Weigert’s Iron Chloride

Working Solution:   Mix equal parts of Sol. I and Sol. II. Recommended staining time is 2 - 5 minutes.

RESULTS:  Nuclei - black; connective tissue - red; cytoplasm - yellowish.

# 8600

Weigert’s Iron Hematoxylin Stain Kit
1 Kit (250 ml Ea. Sol.)

$   158.00

# 8601

Weigert’s Iron Hematoxylin Stain Kit
1 Kit (950 ml Ea. Sol.)

248.00

# 8602

Weigert’s Iron Hematoxylin Stain Kit
1 Kit (3,800 ml Ea. Sol.)

352.00

CONTENTS:   Hematoxylin, Ethanol, Ferric Chloride, Hydrochloric Acid

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

           MALLORY’S ACID HEMATOXYLIN STAIN
(PHOSPHOTUNGSTIC ACID)

A routine stain method for the nervous system because of its ability to demonstrate abnormal or diseased astrocytes. Useful in showing mitochondria, striations of cardiac and voluntary muscle, and as a counterstain for the Periodic Acid-Schiff (PAS) technique. Recommended staining time is 12 - 24 hours.

RESULTS:  Nuclei, mitochondria, fibrin, fibroglia, microglia, and the contractile portions of striated (voluntary) and heart muscle stain blue. Collagen, reticulum, elastin, the ground substances of cartilage and bone stain varying shades of yellow to brownish-red.

# 8650

Mallory’s Acid Hematoxylin Stain
1 Btl. (250 ml)

$   119.00

# 8651

Mallory’s Acid Hematoxylin Stain
1 Btl. (950 ml)

159.00

# 8652

Mallory’s Acid Hematoxylin Stain
1 Pack (Two 950 ml Btls.)

248.00

# 8653

Mallory’s Acid Hematoxylin Stain
1 Gallon (3,800 ml)

345.00

CONTENTS:   Hematoxylin, Phosphotungstic Acid, Potassium Permanganate

This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.

HEIDENHAIN’S IRON HEMATOXYLIN STAIN KIT

Solution I - Ammonio-Ferric Alum
Solution II - 0.5% Aqueous Hematoxylin

A histologic stain composed of ferric salts which ripen and oxidize hematoxylin rapidly, eliminating artificial or natural ripening. Solutions made with iron salts are useful for dense regressive staining. Differentiation can be accurately controlled, and the staining is consistent.

Working Solution:   Dilute Sol. II with an equal quantity of distilled water. Recommended staining time is 1 - 36 hours followed by differentiation in Sol. I.

RESULTS:  Chromatin, nucleoli, mitochondria, centrioles and certain parts of striated muscle fibers are stained black. Other tissue elements are colored by the contrast stain used.

# 8700

Heidenhain’s Iron Hematoxylin Stain Kit
1 Kit (250 ml Ea. Sol.)

$ 141.00

# 8701

Heidenhain’s Iron Hematoxylin Stain Kit
1 Kit (950 ml Ea. Sol.)

201.00

# 8702

Heidenhain’s Iron Hematoxylin Stain Kit
1 Kit (3,800 ml Ea. Sol.)

359.00

CONTENTS:   Ferric Ammonium Sulfate, Hematoxylin, Ethanol

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

EOSIN Y STAINING SOLUTIONS
(AQUEOUS OR ALCOHOLIC)

Solutions are used mainly as background contrast to nuclear stains, i.e., hematoxylin-eosin sequence. Eosin Y stain depicts the cytoplasmic detail of the cell and the tissue structure.

RECOMMENDED PROCEDURE:  Stain 15 seconds to 2 minutes depending upon the depth of counterstain desired.

# 8900

Eosin Y - 1% Aqueous Solution
1 Btl. (250 ml)

$   100.00

# 8901

Eosin Y - 1% Aqueous Solution
1 Btl. (950 ml)

138.00

# 8902

Eosin Y - 1% Aqueous Solution
1 Pack (Two 950 ml Btls.)

196.00

# 8903

Eosin Y - 1% Aqueous Solution
1 Gallon (3,800 ml)

267.00

# 8905

Eosin Y - 0.1% Aqueous Solution
1 Btl. (250 ml)

   82.00

# 8906

Eosin Y - 0.1% Aqueous Solution
1 Pack (Two 250 ml Btls.)

128.00

# 8907

Eosin Y - 0.1% Aqueous Solution
1 Btl. (100 ml)

   47.00

# 8908

Eosin Y - 5% Aqueous Solution
1 Btl. (100 ml)

   63.00

# 8909

Eosin Y - 5% Aqueous Solution
1 Btl. (250 ml)

   102.00

# 8910

Eosin Y - 1% Alcoholic Solution
1 Btl. (250 ml)

100.00

# 8911

Eosin Y - 1% Alcoholic Solution
1 Btl. (950 ml)

150.00

# 8912

Eosin Y - 1% Alcoholic Solution
1 Pack (Two 950 ml Btls.)

228.00

# 8913

Eosin Y - 1% Alcoholic Solution
1 Gallon (3,800 ml)

293.00

CONTENTS:   Eosin Y, Ethanol (where applicable)

These solutions are made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.

EOSIN-PHLOXINE SOLUTION

Solution is used mainly as background contrast to nuclear stains, i.e., hematoxylin-eosin sequence. Stain depicts the cytoplasmic detail of the cell and the tissue structure.

RECOMMENDED PROCEDURE:  Stain 15 seconds to 2 minutes depending upon the depth of counterstain desired.

RESULTS:  Cytoplasm - pink

# 8921

Eosin-Phloxine Solution
1 Btl. (950 ml)

$  177.00

# 8922

Eosin-Phloxine Solution
1 Pack (Two 950 ml Btls.)

243.00

# 8923

Eosin-Phloxine Solution
1 Gallon (3,800 ml)

316.00

# 8924

Eosin-Phloxine Solution
1 Cubtainer (19 L)

796.00

CONTENTS:   Eosin Y, Phloxine, Ethanol, Glacial Acetic Acid

This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.

 

PHLOXINE B COUNTERSTAIN (1% or 0.05%)

Solution is used mainly as background contrast to nuclear stains, i.e., hematoxylin-eosin sequence. Stain depicts the cytoplasmic detail of the cell and the tissue structure.

RECOMMENDED PROCEDURE:  Stain 15 seconds to 2 minutes depending upon the depth of counterstain desired.

RESULTS:  Cytoplasm - pink

# 8930

Phloxine B Counterstain (1%)
1 Btl. (950 ml)

$   171.00

# 8931

Phloxine B Counterstain (1%)
1 Pack (two 950 ml Btls.)

236.00

# 8932

Phloxine B Counterstain (1%)
1 Gallon (3,800 ml)

304.00

# 8935 Phloxine B Counterstain (0.05%)
1 Btl. (950 ml)

     171.00

# 8936 Phloxine B Counterstain (0.05%)
1 Pack (Two 950 ml Btls.)

   236.00

# 8937 Phloxine B Counterstain (0.05%)
1 Gallon (3,800 ml)

   304.00

CONTENTS:   Phloxine, Calcium Chloride

This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.

GOMORI’S TRICHROME STAIN
(HISTOLOGICAL STAIN)

This stain demonstrates fine histological detail of connective tissue, collagen and muscle fibers.

RECOMMENDED PROCEDURE:   Deparaffinize and hydrate to distilled water. Place in Bouin’s solution in oven at 560C for 1 hour. Wash well in running water or until yellow color disappears. Stain nuclei with Weigert’s Iron Hematoxylin for 10 minutes, wash in water, then apply stain for 15 - 20 minutes. Place in 0.5% acetic water for 2 minutes. Dehydrate in 95% ethanol, absolute ethanol, and clear in xylene, 2 changes each.

RESULTS:  Muscle fibers - red; collagen - green; nuclei -blue to black.

# 8990

Gomori’s Trichrome Stain
1 Pack (Two 250 ml Btls.)

$ 228.00

# 9000

Gomori’s Trichrome Stain
1 Pack (Two 950 ml Btls.)

343.00

CONTENTS:   Chromotrope 2R, Light Green SF, Glacial Acetic Acid, Phosphotungstic Acid

This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.

NUCLEAR FAST RED STAIN
(KERNECHTROT)

May be used as a general counterstain in the following procedures: Gridley’s reticulum stain, Snook’s reticulum stain, Lepehne’s method for hemoglobin, Ralph’s method for hemoglobin, Kossa’s method for calcium, Pizzolato’s method, Puchtler-Sweat method for basement membranes, Osmium tetroxide method for fat in frozen sections, Gomori’s method for iron, Mallory’s method for iron, Perl’s method for iron, Tumbull’s Blue method for hemosiderin, Sudan Black B stain for fat, Alcian Blue (short method) with and without hyaluronidase, Mallory’s reaction for iron, and Stain’s bile pigment stain.

RECOMMENDED PROCEDURE:  The staining time depends upon the stain procedure being employed. The time varies from a few seconds to 5 minutes.

RESULTS:  Nuclei - pink to red.

# 9040

Nuclear Fast Red Stain (Kernechtrot)
1 Btl. (250 ml)

$ 177.00

CONTENTS:   Nuclear Fast Red, Aluminum Sulfate

FOR IN VITRO DIAGNOSTIC USE ONLY.

                    POTASSIUM PERMANGANATE (0.5%)

A stain used in histopathology for general laboratory staining procedures.

# 9045

Potassium Permanganate (0.5%)
1 Pack. (Two 250 ml Btls.)

$ 110.00

CONTENTS:   Potassium Permanganate

FOR IN VITRO DIAGNOSTIC USE ONLY.

SCHIFF REAGENT
(FOR PERIODIC ACID-SCHIFF REACTION)

A stain used in histopathology for the staining of glycogen polysaccharides or in combination with the Periodic Acid-Schiff reaction.

RECOMMENDED PROCEDURE:  Stain time required in the Schiff Reagent is 10 - 15 minutes using standard PAS method.

RESULTS:  Glycogen polysaccharides (carbohydrates) stain magenta to purple indicating a " PAS positive".

# 9060

Schiff Reagent
1 Btl. (250 ml)

$ 150.00

# 9061

Schiff Reagent
1 Pack (Two 250 ml Btls.)

236.00

# 9062

Schiff Reagent
1 Pack (Two 950 ml Btls.)

331.00

# 9063

Schiff Reagent
1 Btl. (475 ml)

177.00

CONTENTS:   Basic Fuchsin, Sodium Sulfite, HCl

This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.

SUDAN BLACK B STAIN KIT
(FOR FAT)

Solution I - Sudan Black B
Solution II - Propylene Glycol, 85%
Solution III - Nuclear Fast Red Stain (Kernechtrot)

A staining solution used to stain fat in frozen sections.

RECOMMENDED PROCEDURE:   Dehydrate sections for 5 - 10 minutes in absolute propylene glycol. Stain for 10 - 20 minutes in Sol. I. Differentiate for 2-3 minutes in Sol. III.  Wash in distilled water and counterstain with Sol. III for a few minutes. Wash well and mount.

RESULTS:  Fat - blue-black; nuclei - red.

# 9100

Sudan Black B Stain Kit
1 Kit (250 ml Ea.Sol.)

$ 368.00

# 9110

Solution I - Sudan Black B
1 Pack (Two 250 ml Btls.)

228.00

# 9111 Solution I - Sudan Black B
1 Btl. (250 ml)

    136.00

# 9120

Solution II - Propylene Glycol, ( 85%)
1 Pack (Two 250 ml Btls.)

130.00

# 9121 Solution II - Propylene Glycol, (85%)
1 Btl. (250 ml)

    93.00

# 9131

Solution III - Nuclear Fast Red (Kernechtrot)
1 Btl. (250 ml)

177.00

CONTENTS:   Sudan Black B, Propylene Glycol, Nuclear Fast Red, Aluminum Sulfate

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

GOMORI’S METHOD FOR IRON IN TISSUE WITH NUCLEAR FAST RED OR SAFRANIN
(PRUSSIAN BLUE REACTION)

Solution I - 20% Hydrochloric Acid
Solution II - 10% Potassium Ferrocyanide
Solution III - 0.1% Aqueous Safranin
Solution IIIB - Nuclear Fast Red (Kernechtrot)

Designed to demonstrate deposits of hemosiderin (iron) in tissue specimens.

RECOMMENDED PROCEDURE: Working Solution:  Mix just prior to use, 50 ml of Sol. I with 50 ml of Sol. II. Run control slide. Deparaffinize and hydrate to distilled or deionized water. Cover with working solution for 20 - 30 minutes. Rinse in distilled or deionized water, counterstain with Sol. III or IIIB for 3 - 5 minutes, and rinse again. Dehydrate in 95% ethanol, absolute ethanol - 2 changes, clear in xylene - 2 changes, and mount.           NOTE: Chemically clean glassware and plastic-tipped or paraffin-coated forceps are suggested.

RESULTS: Iron pigments - bright blue; nuclei - red; cytoplasm - light pink.

# 9139 Gomori’s Method for Iron in Tissue with Solution IIIB- Nuclear Fast Red
1 Kit (250 ml Ea. Sol.)

$ 330.00

# 9140

Gomori’s Method for Iron in Tissue with Solution IIIA - Safranin
1 Kit (250 ml Ea. Sol.)

236.00

# 9141 Solution I - Hydrochloric Acid (20%)
1 Btl. (250 ml)

    100.00

# 9142 Solution II - Potassium Ferrocyanide (10%)
1 Btl. (250 ml)

    132.00

# 9143 Solution IIIA - Aqueous Safranin (0.1%)
1 Btl. (250 ml)

    82.00

# 9144 Solution IIIB - Nuclear Fast Red
1 Btl. (250 ml)

     177.00

CONTENTS:  HCl, Potassium Ferrocyanide, Safranin, Nuclear Fast Red, Aluminum Sulfate

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

STAT STAIN FOR FROZEN SECTIONS
(TOLUIDINE BLUE-BASIC FUCHSIN)

A polychromatic solution designed for the rapid staining of frozen sections.

RECOMMENDED PROCEDURE:  Float section onto slide and cover with stain. Immediately rinse slide with tap water. Dehydrate, clear and mount.

RESULTS:  Blue nuclei against a red cytoplasm.

# 9150

Stat Stain for Frozen Sections
1 Btl. (100 ml)

$ 134.00

# 9151

Stat Stain for Frozen Sections
1 Pack (Two 100 ml Btls.)

211.00

# 9152

Stat Stain for Frozen Sections
1 Btl. (500 ml)

421.00

CONTENTS:   Toluidine Blue, Basic Fuchsin, Ethanol

This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.

MAYER’S MUCICARMINE STAIN KIT
(FOR CRYPTOCOCCUS)

Solution I - (Solution A - Weigert’s Hematoxylin, Solution B - Weigert’s Iron Chloride)
Solution II - Mayer’s Mucicarmine Stain
Solution III - Metanil Yellow Stain

RECOMMENDED PROCEDURE:  Working Solution I: Mix equal parts of Sol. A with Sol. B. Working Solution II: Dilute 1 part Sol. II with 4 parts tap water or less for greater intensity in staining. Deparaffinize and hydrate to distilled water. Apply Working Sol. I - 1-7 minutes. Wash in running water - 10 minutes. Stain in Working Sol. II - 1 hour. Rinse quickly in distilled water. Stain in Sol. III - 1 minute and rinse again. Dehydrate and clear in xylene, 2 changes each.  NOTE:  Carminophilic properties will be obscured if overstained with Working Sol. I.

RESULTS:  Capsule of Cryptococcus and mucin stain deep rose to red; nuclei stain black.

# 9155

Mayer’s Mucicarmine Stain Kit
1 Kit (250 ml Ea. Sol.)

$ 236.00

# 9158

Solution III - Metanil Yellow
1 Btl. (250 ml)

$ 134.00

CONTENTS:    Ethanol, Hematoxylin, Ferric Chloride, HCl, Carmine, Aluminum Chloride, Metanil Yellow, Acetic Acid

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

MAYER’S MUCICARMINE STAIN

A stain designed to show mucin derived from epithelial cells (bronchi and colon).

RECOMMENDED PROCEDURE:  Stain may be used straight or diluted at 1:4 - 1:10 with distilled water or 50% to 70% alcohol. The alcoholic solution is preferable. Stain sections for 15 minutes or longer. Wash quickly in distilled water. Dehydrate, clear and mount.

RESULTS:  Mucin stains red.

# 9160

Mayer’s Mucicarmine Stain
1 Btl. (100 ml)

$   134.00

# 9161

Mayer’s Mucicarmine Stain
1 Pack (Two 100 ml Btls.)

211.00

# 9162

Mayer’s Mucicarmine Stain
1 Pack (Two 250 ml Btls.)

281.00

# 9163

Mayer’s Mucicarmine Stain
1 Btl. (475 ml)

237.00

CONTENTS:   Carmine, Aluminum Chloride

This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.

BENNHOLD’S CONGO RED STAIN KIT
(FOR AMYLOID)

Solution I - Congo Red
Solution II - Lithium Carbonate (Saturated Aqueous)
Solution III - Ethanol (80%)
Solution IV - Harris’ Alum Hematoxylin

A simple and reliable procedure which provides a permanent stain for amyloid.

RECOMMENDED PROCEDURE:  Fix in absolute alcohol or 10% formalin (Product # 9700). Stain in Sol. I for 10 - 20 minutes or longer if a deeper color is desired. Dip for 15 seconds in Sol. II. Decolorize with Sol. III until stain no longer washes out. Wash in running water for 15 minutes. Counterstain 1 - 8 minutes in Sol. IV. Wash in tap water, dehydrate, clear and mount.

RESULTS:  Amyloid - red; nuclei - blue; hyalin and colloid - unstained.

# 9200 Bennhold’s Congo Red Stain Kit
1 Kit (250 ml Ea. Sol.)

$ 315.00

# 9210

Solution I - Congo Red
1 Pack (Two 250 ml Btls.)

171.00

# 9220

Solution II - Lithium Carbonate (Saturated Aqueous)
1 Pack (Two 250 ml Btls.)

100.00

# 9230 Solution III - Ethanol (80%)
1 Pack (Two 250 ml Btls.)

100.00

# 9240

Solution IV - Harris’ Alum Hematoxylin
1 Pack (Two 250 ml Btls.)

167.00

CONTENTS:   Congo Red, Lithium Carbonate, Ethanol, Hematoxylin, Potassium Alum, Mercuric Oxide (Red)

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

VERHOEFF’S ELASTIC TISSUE STAIN KIT

Solution I - Alcoholic Hematoxylin
Solution II - Aqueous Ferric Chloride (10%)
Solution III - Iodine
Solution IV - Aqueous Ferric Chloride (2%)
Solution V - Aqueous Phloxine (0.5%)
Used to stain elastic fibers, nuclei and collagen.

RECOMMENDED PROCEDURE:  Working Solution:  In the following order, mix 20 ml of Sol. I + 8 ml of Sol. II + 8 ml of Sol. III. NOTE:  This staining solution should,be used within 24 hours. Immerse sections in working solution for 15 minutes to 1 hour until perfectly black. Differentiate in Sol. IV for a few minutes. Examine sections in water under low magnification to observe the stages in differentiation. If the differentiation has been carried too far, (provided they have not been treated with alcohol) the sections may be restained. Wash in water. Place in 95% alcohol to remove iodine and leave sections in tap water       5 minutes or longer. Counterstain 3 - 4 minutes in Sol. V. Clear in xylene and mount.

RESULTS:  Elastic fibers - intensely blue-black; nuclei - blue to black; collagen - red.

# 9250 Verhoeff’s Elastic Tissue Stain Kit
1 Kit (250 ml Ea. Sol.)

$ 280.00

# 9260

Verhoeff’s Elastic Tissue Stain Kit
1 Kit (950 ml Ea. Sol.)

479.00

# 9270

Solution I - Alcoholic Hematoxylin
1 Pack (Two 950 ml Btls.)

241.00

# 9280 Solution II - Aqueous Ferric Chloride (10%)
1 Pack (Two 950 ml Btls.)

171.00

# 9290

Solution III - Iodine
1 Pack (Two 950 ml Btls.)

201.00

# 9300 Solution IV - Aqueous Ferric Chloride (2%)
1 Pack (Two 950 ml Btls.)

150.00

# 9310

Solution V -   Aqueous Phloxine (0.5%)
1 Pack (Two 950 ml Btls.)

171.00

CONTENTS:   Hematoxylin, Ethanol, Ferric Chloride, Iodine, Potassium Iodide, Phloxine

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

MALLORY’S ANILINE BLUE COLLAGEN STAIN KIT

Solution I - Aniline Blue
Solution II - 0.5% Acid Fuchsin

Demonstrates collagenous fibrils, cartilage, bone, amyloid, and nuclei.

RECOMMENDED PROCEDURE:  Sections should be Zenker-fixed and procedure for removal of pigments and precipitates should be followed prior to staining. Stain with       Sol. II for 1 - 5 minutes or longer. (Omit Sol. II to highlight collagenous fibrils). Transfer directly to Sol. I without washing in water. Stain for 20 - 60 minutes or longer. Dehydrate, clear and mount. For celloidin sections, shorten staining time, decolorize, dehydrate in 95% ethanol and clear by the blotting paper xylol method or in terpineol and mount in balsam.

RESULTS:  Ground substances of cartilage and bone, mucus, amyloid - varying shades of blue; nuclei, fibroglia, myoglia and neuroglia fibrils, axis cylinders and fibrin -red; erythrocytes and myelin - yellow; elastic fibrils - pale pink, pale yellow or unstained.

# 9330

Mallory’s Aniline Blue Collagen Stain Kit
1 Kit (250 ml Ea. Sol.)

$  211.00

# 9340

Mallory’s Aniline Blue Collagen Stain Kit
1 Kit (950 ml Ea. Sol.)

306.00

# 9350

Solution I - Aniline Blue
1 Pack (Two 950 ml Btls.)

324.00

# 9360

Solution II - 0.5 % Acid Fuchsin
1 Pack (Two 950 ml Btls.)

236.00

CONTENTS:  Aniline Blue, Orange G, Phosphotungstic Acid, Acid Fuchsin

These solutions are made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.

NEISSER STAIN KIT

Solution IA - Methylene Blue
Solution IB - Crystal Violet 
                                                                                        Solution II - Bismarck Brown

RECOMMENDED PROCEDURE:  Specimens - intestinal (calciform cells), tracheal and embryonic tissue. Stain in Sol. I for 5 - 10 minutes. Rinse in 95% Ethanol. Transfer to     Sol. II until the slide appears dark green. Dehydrate, clear and mount in balsam.  Mix two parts of Solution IA - Methylene Blue with one part Solution IB - Crystal Violet.  Place stain mixture onto slide for 10 - 15 seconds.  Afterwards, allow the excess stain to run off the slide.  Add Bismarck Brown for 45 seconds.  Rinse slide with tap water.  Allow slide to dry and then view.

RESULTS:  Cartilage - dark brown; mucin - light brown; nuclei of all the cells - green.  Examine under oil immersion at 1000X with direct illumination.  Blue violet is positive (either entire cell or intracellular granules);  yellow - brown is negative.

# 9363

Neisser Stain Kit
1 Kit (250 ml Ea. Sol.)

$   228.00

CONTENTS:   Bismarck Brown, Methyl Green   Methylene Blue, Crystal Violet, Bismarck Brown, Glacial Acetic Acid, Ethanol.

These solutions are made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.

BISMARCK BROWN WITH METHYL GREEN STAIN KIT

Solution I - 1% Aqueous Bismarck Brown
Solution II - 0.5% Aqueous Methyl Green

RECOMMENDED PROCEDURE:  Specimens - intestinal (calciform cells), tracheal and embryonic tissue. Stain in Sol. I for 5 - 10 minutes. Rinse in 95% Ethanol. Transfer to     Sol. II until the slide appears dark green. Dehydrate, clear and mount in balsam.

RESULTS:  Cartilage - dark brown; mucin - light brown; nuclei of all the cells - green.

# 9370

Bismarck Brown with Methyl Green Stain Kit
1 Kit (250 ml Ea. Sol.)

$   211.00

# 9380

Bismarck Brown with Methyl Green Stain Kit
1 Kit (950 ml Ea. Sol.)

342.00

# 9390

Solution I - 1% Aqueous Bismarck Brown
1 Pack (Two 950 ml Btls.)

284.00

# 9400

Solution II - Aqueous Methyl Green (0.5%)
1 Pack (Two 950 ml Btls.)

284.00

CONTENTS:   Bismarck Brown, Methyl Green

These solutions are made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.

 METHYL GREEN WITH PYRONIN STAIN

Unna Pappenheim Method modified by Saathof for bacteria, DNA and RNA, and plasma cells.

RECOMMENDED PROCEDURE:  Bacteria: Fixative - absolute alcohol or Carnoy’s fluid. Stain 2 - 4 minutes (up to 30 minutes if necessary). Wash in distilled water. Dehydrate quickly in absolute alcohol or acetone. Clear in xylol and mount in balsam. Celloidin sections are cleared in terpineol and mounted in balsam.

RESULTS:  Bacteria - bright red; chromatin - green; all other structures stain varying shades of red.

RECOMMENDED PROCEDURE: DNA and RNA:  Fixative - Clarke and Camoy’s fluid. Stain 10 minutes. Rinse twice in distilled water briefly. Blot with smooth filter paper.

Differentiation Solution:  Mix 30 ml Tertiary Butyl Alcohol with 10 ml 100% Alcohol. Dehydrate in a fresh change of the differentiating solution for 1 - 2 minutes. (Slides may be left in solution for 4 - 5 minutes) Clear in two changes of xylene for 10 minutes each.

RESULTS:  DNA - blue green; RNA - bright red; remainder of tissue - pale pink.

RECOMMENDED PROCEDURE:  Plasma cells:  Fixative - absolute alcohol or Camoy’s fluid. Stain 20 minutes. Rinse rapidly in water. Differentiate and dehydrate rapidly in absolute alcohol. Clear in xylol.

RESULTS:  Nuclei - green; cytoplasm - deep red.

# 9410

Methyl Green with Pyronin Stain
1 Btl. (250 ml)

$  171.00

# 9411

Methyl Green with Pyronin Stain
1 Pack (Two 250 ml Btls.)

228.00

# 9412

Methyl Green with Pyronin Stain
1 Pack (Two 950 ml Btls.)

404.00

# 9425

Methyl Green, 2% Aqueous
1 Btl. 100 ml)

103.00

CONTENTS:  Methyl Green, Pyronin, Ethanol, Glycerol, Phenol

This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.

NILE BLUE A STAIN
(BASIC BLUE 12; NILE BLUE SULFATE; NIBLAU BX)

A stain used in histology for the differentiation of melanins and lipofuscins.

RECOMMENDED PROCEDURE:   Deparaffinize and hydrate to distilled water. Stain 20 minutes. Wash 10 - 20 minutes in running water. Mount in glycerol gelatin.

RESULTS:  Lipofuscins - dark blue or green - blue; melanins - dark green. Cytoplasms: muscle - pale green, red corpuscles - greenish yellow to greenish blue, myelin - green to deep blue, nuclei - not stained.

# 9440

Nile Blue A Stain
1 Btl. (250 ml)

$  171.00

# 9441

Nile Blue A Stain
1 Pack (Two 250 ml Btls.)

248.00

CONTENTS:   Nile Blue A, Sulfuric Acid

This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.

HERXHEIMER’S SUDAN IV STAIN KIT
(FOR FAT)

Solution I - Sudan IV (Scarlet Red)
Solution II - Ethanol (70%)
Solution III - Mayer’s Acid Alum Hematoxylin

Sudan IV is more soluble in fat than in alcohol allowing fat to absorb the stain.

RECOMMENDED PROCEDURE:  NOTE:   Keep Sol. I tightly stoppered since the evaporation of the alcohol and acetone causes precipitation of the stain. Dip for an instant in Sol. II. Stain in Sol. I for 2 - 5 minutes. Wash quickly in Sol. II and transfer to distilled water. Counterstain in Sol. III. Wash thoroughly in water and mount in glycerin.

RESULTS:  Nuclei - blue; fat - orange to red; normal myelin and fatty acids - unstained.

# 9460

Herxheimer’s Sudan IV Stain Kit
1 Kit (250 ml Ea. Sol.)

$ 320.00

# 9470

Solution I - Sudan IV (Scarlet Red)
1 Pack (Two 250 ml Btls.)

190.00

# 9480

Solution II - Ethanol (70%)
1 Pack (Two 250 ml Btls.)

82.00

# 9490

Solution III - Mayer’s Acid Alum Hematoxylin
1 Pack (Two 250 ml Btls.)

190.00

CONTENTS:   Sudan IV, Ethanol, Acetone, Hematoxylin, Citric Acid, Sodium Iodate, Potassium Alum

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

MALDONADO’S METHOD STAIN KIT
(FOR PANCREATIC ISLET CELLS)

Solution I - Phloxine (1%)
Solution II - Phosphotungstic Acid (3%)
Solution III - Azure II (0.05%)
Solution IVA - Weigert’s Hematoxylin
Solution IVB - Weigert’s Iron Chloride

RECOMMENDED PROCEDURE:  Prepare working Solution IV:  Mix equal parts of Sol. IVA with Sol. IV B. Sol. I -10 minutes. Rinse in distilled water. Sol. II - 1 minute. Rinse again. Sol. III - 30 seconds. Rinse again. Sol. IV - 1 minute. Rinse in distilled water. Dehydrate and clear in xylene, 2 changes each.

RESULTS:  A cells - purple; B cells - violet blue; D cells - light blue with evident granules; exocrine cells - grayish blue with red granules.

# 9500

Maldonado’s Method Stain Kit
1 Kit (250 ml Ea. Sol.)

$ 316.00

# 9501

Solution I - Phloxine (1%)
1 Pack (Two 250 ml Btls.)

130.00

# 9502

Solution II - Phosphotungstic Acid (3%)
1 Pack (Two 250 ml Btls.)

146.00

# 9503

Solution III -  Azure II (0.05%)
1 Pack (Two 250 ml Btls.)

130.00

# 9504

Solution IVA - Weigert’s Hematoxylin
1 Pack (Two 250 ml Btls.)

153.00

# 9505

Sol. IVB - Weigert’s Iron Chloride
1 Pack (Two 250 ml Btls.)

130.00

CONTENTS:   Phloxine, Phosphotungstic Acid, Azure II, Hematoxylin, Ferric Chloride, Ethanol, HCl

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

BERG’S METHOD STAIN KIT
(FOR SPERMATOZOA IN SECTIONS)

Solution I - Carbol Fuchsin (Putt’s)
Solution II - Saturated Lithium Carbonate
Solution III - Glacial Acetic Acid (5% Alcoholic)
Solution IV - Methylene Blue (0.5%)

RECOMMENDED PROCEDURE:  Gently agitate slides to avoid uneven staining. Sol. I - 3 minutes. Sol. II - 1 minute. Decolorize in Sol. III - 5 minutes. Absolute alcohol - 1 minute, 2 changes each. Sol. IV - 45 seconds. Rinse in absolute alcohol, 2 changes each and clear in xylene, 2 - 3 changes each.

RESULTS:  Spermatozoa - brilliant red; red cells - pale pink; other tissue            components - blue to purple.

# 9510

Berg’s Method Stain Kit
1 Kit (250 ml Ea. Sol.)

$ 304.00

CONTENTS:   Basic Fuchsin, Phenol, Ethanol, Lithium Carbonate, Glacial Acetic Acid, Methylene Blue

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

DIAZO METHOD STAIN KIT
(FOR ARGENTAFFIN GRANULES)

Solution I - Brentamine Fast Red B (1% Stock)
Solution II - Lithium Carbonate (Stock)
Solution III - Mayer’s Acid Alum Hematoxylin

Argentaffin cells or Kultschitzsky cells occur normally in the mucosa of the intestines, from the stomach to the large bowel. Occasionally, they give rise to tumors known as "carcinoid" tumors which have a characteristic yellow color. These tumors are most common in the distal one-third of the appendix where they generally lead to secondary appendicitis.

RECOMMENDED PROCEDURE: Working Solution:  Mix 5.0 ml of Sol. I with 2.0 ml of Sol. II. Incubate for 6 - 8 minutes at 40C. Deparaffinize section and hydrate to distilled water. Use working solution at 40C for 40 - 60 seconds. Sol. III - 3 minutes. Blue in warm tap water - 5 minutes. Rinse in 95% ethanol, 2 changes each. Dehydrate rapidly in absolute ethanol, then clear in xylene, 2 changes each.

RESULTS:  Argentaffin granules - rust red; background - yellow; nuclei - blue.

# 9520

Diazo Method Stain Kit
1 Kit (250 ml Ea. Sol.)

$   248.00

CONTENTS:   Brentamine Fast Red B, Lithium Carbonate, Hematoxylin, Citric Acid, Sodium Iodate, Potassium Alum

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

LENDRUM’S STAIN KIT
(FOR INCLUSION BODIES IN SECTIONS)

Solution I - Mayer’s Acid Alum Hematoxylin
Solution II - Phloxine Solution 0.5%
Solution III - Tartrazine Solution 2.5%

Suitable for the demonstration of intranuclear inclusion bodies of chickenpox, Herpes Zoster (shingles), and the intracytoplasmic Guarneri bodies of smallpox.

RECOMMENDED PROCEDURE:  Sol. I - 5 - 10 minutes. Blue in running water - 15 minutes. Sol. II - 30 min. Rinse briefly in distilled water. Differentiate in Sol. III until inclusion bodies stand out bright red on a yellow background. Dehydrate in 95% ethanol, absolute ethanol, and clear in xylene, 2 changes each.

RESULTS:  Inclusion bodies - red; nuclei - blue; background - yellow.

# 9540

Lendrum’s Stain Kit
1 Kit (250 ml Ea. Sol.)

$ 248.00

CONTENTS:   Hematoxylin, Potassium Alum, Sodium Iodate, Citric Acid, Phloxine, Ethanol, Calcium Chloride, Tartrazine

These solutions are made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.

PAGE-GREEN METHOD STAIN KIT
(FOR INCLUSION BODIES)

Solution I - Harris’ Alum Hematoxylin
Solution II - Acid Alcohol (1%)
Solution III - Shorr-Single Differential Stain

Technique used in the staining of distemper inclusion bodies.

RECOMMENDED PROCEDURE:  Sol. I - 3 - 5 minutes. Rinse in water. Differentiate in Sol. II until cytoplasm is free of hematoxylin (check microscopically). Blue in ammonia water. Wash in running water -10 minutes. Sol. III - 1 minute. Rinse in 95% ethanol (check microscopically for clear, light-green connective tissue.) Dehydrate in 95% ethanol, absolute ethanol, and clear in xylene, 2 changes each.

RESULTS:  Inclusion bodies - brilliant red; connective tissue - light green; elastic tissue - purplish red; muscle - red; keratin - orange; erythrocytes - orange red; nuclei - blue.

# 9560

Page-Green Method Stain Kit
1 Kit (250 ml Ea. Sol.)

$ 248.00

CONTENTS:   Hematoxylin, Potassium Alum, Ethanol, HCl, Biebrich Scarlet, Orange G, Fast Green FCF, Phosphotungstic Acid, Phosphomolybdic Acid, Glacial Acetic Acid

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

    GRAM STAIN KIT  
WITH SAFRANIN OR CARBOL FUCHSIN COUNTERSTAIN

(FOR BACTERIA IN TISSUE)

Solution I - Crystal Violet
Solution II - Grams Iodine
Solution III - Acetone Alcohol
Solution IV - Safranin
Solution IV - Carbol Fuchsin

RECOMMENDED PROCEDURE:  Bring thin paraffin sections to water (6 microns or less). Flood sections with Sol. I for 1 minute. Rinse in water. Wash in Sol. II; then flood with    Sol. II for 1 - 2 minutes. Decolorize with Sol. III until the purple color no longer runs off (approximately 20 - 30 seconds). Wash in water. Counterstain with Sol. IV or IVB for        30 seconds. Rinse in water. Blot dry and dehydrate rapidly in a mixture of 2 parts aniline and 1 part xylol, or ALLOW TO AIR DRY THOROUGHLY.  Clear in 2 changes of xylol and mount.

RESULTS:  Gram-positive bacteria, many fungi and fibrin - blue-black to violet. Gram-negative bacteria - red. Cytoplasm - pink.

# 9570

Gram Stain Kit with Safranin Counterstain
1 Kit (250 ml Ea. Sol.)

$    195.00

# 9571

Solution  I - Crystal Violet
1 Btl. (250 ml)

65.00

# 9572

Solution II - Grams Iodine
1 Btl. (250 ml)

 82.00

# 9573

Solution III - Acetone Alcohol
1 Btl. (250 ml)

82.00

# 9574

Solution IV - Safranin
1 Btl. (250 ml)

65.00

# 9575

Gram Stain Kit with Carbol Fuchsin Counterstain
1 Kit (250 ml Ea. Sol.)
    229.00

# 9576

Solution I - Crystal Violet
1 Btl. (250 ml)
      65.00

# 9577

Solution II - Grams Iodine
1 Btl. (250 ml)
      82.00

# 9578

Solution III - Acetone Alcohol
1 Btl. (250 ml)
      82.00

# 9579

Solution IVB - Carbol Fuchsin
1 Btl. (250 ml)
      110.00

CONTENTS:   Crystal Violet, Iodine, Potassium Oxalate, Ethanol, Acetone, Safranin, Basic Fuchsin, Phenol

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

FITE’S METHOD STAIN KIT
(FOR ACID-FAST BACTERIA IN TISSUE)

Solution I - Ziehl-Neelsen Carbol Fuchsin
Solution II - 1% Sulfuric Acid
Solution III - Loeffler’s Methylene Blue

RECOMMENDED PROCEDURE:   Deparaffinize through (1 part peanut oil - 2 parts xylene) 2 changes, 12 minutes each. Drain, wipe off excess oil and blot to opacity. The residual oil helps prevent shrinkage and injury of section. Sol. I - 30 minutes at room temperature. Wash in tap water - 3 minutes. Differentiate slides individually with Sol. II until sections are faint pink. Wash in tap water - 3 minutes. Sol. III - 30 seconds. Rinse in water, blot and ALLOW TO AIR DRY THOROUGHLY.  Before mounting, dip slide in xylene.

RESULTS:  Acid-fast bacilli - red; Lepra bacilli - red; Norcardia filaments - red;        background - pale blue.

# 9580

Fite’s Method Stain Kit
1 Kit (250 ml Ea. Sol.)

$ 171.00

CONTENTS:   Basic Fuchsin, Phenol, Ethanol, Sulfuric Acid, Methylene Blue

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

GRIDLEY’S METHOD STAIN KIT
(FOR ENDAMOEBA HISTOLYTICA)

Solution I - Harris’ Alum Hematoxylin
Solution II - 1% Acid Alcohol
Solution III - Aniline Eosin
Solution IV - Napthol Green B

Product designed to demonstrate ingested erythrocytes in the trophozoites of E. histolytica.

RECOMMENDED PROCEDURE:   Sol. I - 10 minutes. Wash in running water 5 minutes. Differentiate in Sol. II. Wash in running water - 5 minutes. Blue in ammonia water (5 - 10 drops of ammonia to 100 ml of distilled water.) Wash in running water - 5 minutes. Sol. III - 5 minutes. Rinse in distilled water. (Note deep rose color.) Sol. IV - 5 minutes. Differentiate in 95% Alcohol, 2 changes, until erythrocytes in section are bright rose    (check with microscope). Dehydrate in absolute alcohol and clear in xylene, 2 changes each.

RESULTS:  Amoeba - blue-green; nuclei - deep blue green; ingested RBC’s - deep rose; tissue - green.

# 9600

Gridley’s Method Stain Kit
1 Kit (250 ml Ea. Sol.)

$ 253.00

CONTENTS:   Hematoxylin, Potassium Alum, Mercuric Oxide (Red), Ethanol, HCl, Eosin Y, Aniline, Glacial Acetic Acid, Napthol Green B

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

UNNA STAIN
(FOR MAST CELLS)

A polychromatic stain used in histology for the differentiation of mast cells from plasma cells.

RECOMMENDED PROCEDURE:  Deparaffinize and hydrate to distilled water. Stain 10 minutes. Rinse in distilled water. Differentiate in glycerine-ether solution, diluted 5 to 10 times with distilled water for 30 seconds to 1 minute, or until medium blue. Wash thoroughly in water for 2 to 5 minutes, then blot. Dehydrate and clear in xylene 2-3 changes and mount.

RESULTS:  Mast cell granules stain red while others stain greenish blue.

# 9620 Unna Stain
1 Pack (Two 100 ml Btls.)

$ 190.00

# 9621 Unna Stain
1 Btl. (100 ml)

    119.00

CONTENTS:  Methylene Blue, Ethanol, Potassium Carbonate

This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.

AQUEOUS NIGROSIN SOLUTIONS
(1%, 10%)

Nigrosin is used as a background stain.  It adds contrast for better visualization of staining.

RECOMMENDED PROCEDURE:  Mix specimen with Aqueous Nigrosin solution and allow to dry with or without heat.

RESULTS:  Dark background is obtained which allows for improved visualization.

# 9624 Aqueous Nigrosin, 1% Aqueous
1 Btl.  (100 ml)

$  55.00

# 9625 Aqueous Nigrosin, 1% Aqueous
1 Btl.  (250 ml)

  100.00

# 9626 Aqueous Nigrosin, 10% Aqueous
1 Btl. (250 ml)

    143.00

# 9627 Aqueous Nigrosin, 10% Aqueous
1 Btl. (100 ml)

    83.00

CONTENTS:  Nigrosin, Distilled water.

This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.

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