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METHANOL FIXATIVE

For use as a fixative prior to staining bloody specimen material or blood culture supematant fluid. Methanol preserves the morphology of red blood cells as well as bacteria.

RECOMMENDED PROCEDURE:  Place material to be stained on a clean glass slide. Flood slide with methanol fixative for 1 minute. Drain without rinsing and allow slide to air dry prior to staining.

# 5994 Methanol Fixative
1 Btl.  (475 ml)
$ 56.00
# 5995 Methanol Fixative
1 Pack (Two 250 ml Btls.)
65.00
# 5996 Methanol Fixative
1 Pack (Four 250 ml Btls.)
 74.00
# 5997 Methanol Fixative
1 Pack (Two 950 ml Btls.)
82.00
# 5998 Methanol Fixative
1 Gallon (3,800 ml)
100.00
# 5999 Methanol Fixative
1 Case (Four 3,800 ml Btls.)
217.00

CONTENTS:  Methanol

FOR IN VITRO DIAGNOSTIC USE ONLY.

GRAM STAIN KIT
(FOR DIFFERENTIAL STAINING OF BACTERIA)

Solution I - Crystal Violet
Solution II - Grams Iodine
Solution III - Ethanol (70%)
Solution IIIA - Ethanol (95%)
Solution IIIB - Acetone-Alcohol
Solution IV - Safranin
Solution IVA - Basic Fuchsin
Solution IVB - Carbol Fuchsin

AVAILABLE IN VARIOUS COUNTERSTAINS AS FOLLOWS:

Safranin - Routinely used for gram negative bacteria.

Basic Fuchsin - More intense overall staining to clearly identify weak gram-negative bacteria and also aids in the detection of Bordatella.

Carbol Fuchsin - A counterstain of greater overall intensity to easily identify Legionella.

Also available with various decolorizers to suit individual preference of technique. Convenient flip-top cap for 250 ml and 950 ml sizes.

RECOMMENDED PROCEDURE:  Cover fixed smear with Sol. I for 1 minute and rinse gently with distilled water. Apply Sol. II for 1 minute and rinse gently with distilled water. Decolorize with Sol. III, Sol. IIIA, or Sol. IIIB until the purple color no longer runs off the slide, (from 2 - 20 seconds depending upon decolorizer and thickness of smear) and rinse with distilled water. Counterstain with Sol. IV, Sol. IVA or Sol. IVB for 1 minute and rinse with distilled water. Allow to dry and examine under oil immersion.

NOTE:  For a faster decolorizer, use Sol.IIIB - Acetone-Alcohol. For a more intense counterstain, we recommend Sol. IVA - Basic Fuchsin or Sol. IVB - Carbol Fuchsin.

RESULTS:  Gram-positive bacteria stain purple to blue black. Gram-negative bacteria stain pink to red.

# 6000 Gram Stain Kit (Sol. III)
1 Kit (250 ml Ea. Sol.)
$ 82.00
# 6050

Gram Stain Kit (Sol. IIIA)
1 Kit (250 ml Ea. Sol.)

82.00

# 6060

Gram Stain Kit (Sol. IIIB)
1 Kit (250 ml Ea. Sol.)

82.00

# 6070

Gram Stain Kit (Sol. III, Sol. IVA)
1 Kit (250 ml Ea. Sol.)

87.00

# 6080

Gram Stain Kit (Sol. IIIA, Sol. IVA)
1 Kit (250 ml Ea. Sol.)

87.00

# 6090

Gram Stain Kit (Sol. IIIB, Sol. IVA)
1 Kit (250 ml Ea. Sol.)

87.00

# 6095

Gram Stain Kit (Sol. III, Sol. IVB)
1 Kit (250 ml Ea. Sol.)

107.00

# 6096

Gram Stain Kit (Sol. IIIA, Sol. IVB)
1 Kit (250 ml Ea. Sol.)

107.00

# 6097 Gram Stain Kit (Sol. IIIB, Sol. IVB)
1 Kit (250 ml Ea. Sol.)

107.00

# 6100

Solution I - Crystal Violet
1 Pack ( Four 250 ml Btls.)

74.00

# 6120

Solution I - Crystal Violet
1 Pack (Two 950 ml Btls.)

110.00

# 6150

Solution I - Crystal Violet
1 Gallon (3,800 ml)

177.00

# 6151

Solution I - Crystal Violet (Slim-line Btl.)
1 Gallon (3,800 ml)

193.00

# 6200

Solution II - Grams Iodine
1 Pack (Four 250 ml Btls.)

82.00

# 6220

Solution II - Grams Iodine
1 Pack (Two 950 ml Btls.)

122.00

# 6250

Solution II - Grams Iodine
1 Gallon (3,800 ml)

195.00

# 6251

Solution II - Grams Iodine (Slim-line Btl.)
1 Gallon (3,800 ml)

207.00

# 6260

Solution IIIB - Acetone-Alcohol
1 Pack (Four 250 ml Btls.)

88.00

# 6270

Solution IIIB - Acetone-Alcohol
1 Pack (Two 950 ml Btls.)

137.00

# 6280

Solution IIIB - Acetone-Alcohol
1 Gallon (3,800 ml)

161.00

# 6281

Solution IIIB - Acetone-Alcohol (Slim-line Btl.)
1 Gallon (3,800 ml)

182.00

# 6300

Solution III - Ethanol (70%)
1 Pack (Four 250 ml Btls.)

87.00

# 6320

Solution III - Ethanol (70%)
1 Pack (Two 950 ml Btls.)

137.00

# 6350

Solution III - Ethanol (70%)
1 Gallon (3,800 ml)

161.00

# 6351

Solution III - Ethanol (70%) (Slim-line Btl.)
1 Gallon (3,800 ml)

182.00

# 6360

Solution IIIA - Ethanol (95%)
1 Pack (Four 250 ml Btls.)

88.00

# 6370

Solution IIIA - Ethanol (95%)
1 Pack (Two 950 ml Btls.)

141.00

# 6380

Solution IIIA - Ethanol (95%)
1 Gallon (3,800 ml)

162.00

# 6381

Solution IIIA - Ethanol (95%) (Slim-line Btl.)
1 Gallon (3,800 ml)

182.00

# 6400

Solution IV - Safranin
1 Pack (Four 250 ml Btls.)

74.00

# 6420

Solution IV - Safranin
1 Pack (Two 950 ml Btls.)

130.00

# 6450

Solution IV - Safranin
1 Gallon (3,800 ml)

177.00

# 6451

Solution IV - Safranin (Slim-line Btl.)
1 Gallon (3,800 ml)

193.00

# 6455

Solution IVA - Basic Fuchsin
1 Pack (Four 250 ml Btls.)

96.00

# 6456

Solution IVA - Basic Fuchsin
1 Pack (Two 950 ml Bts.)

171.00

# 6457

Solution IVA - Basic Fuchsin
1 Gallon (3,800 ml)

306.00

# 6458

Solution IVA - Basic Fuchsin (Slim-line Btl.)
1 Gallon (3,800 ml)

318.00

# 6460

Solution IVB - Carbol Fuchsin
1 Pack (Four 250 ml Btls.)

173.00

# 6461

Solution IVB - Carbol Fuchsin
1 Pack (Two 950 ml Btls.)

226.00

# 6462

Solution IVB - Carbol Fuchsin
1 Gallon (3,800 ml)

388.00

# 6463

Solution IVB - Carbol Fuchsin (Slim-line Btl.)
1 Gallon (3,800 ml)

415.00

CONTENTS:  Crystal Violet, Ethanol, Potassium Iodide, Iodine, Acetone, Safranin, Basic Fuchsin, Phenol

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

GRAM STAIN KIT
KOPELOFF'S MODIFICATION
(WITH SAFRANIN COUNTERSTAIN)

Solution IA - Crystal Violet
Solution IB - Sodium Bicarbonate, 5% (wt/vol)
Solution II  - Iodine
Solution III  - Acetone Alcohol
Solution IV - Safranin
                                                                                                                              SUMMARY AND EXPLANATION:                                                                                        The Kopeloff's Modification of the traditional Gram Stain technique.

RECOMMENDED PROCEDURE FOR OPTIMUM RESUTLS:                                                    1.  Cover the fixed slide with Solution IA and then add 5 drops of Sodium Bicarbonate and blow on slide to mix.  Let stand for 15 seconds.  Continue staining as usual for Gram Stain.  Wash off gently with distilled water.                                                                                                NOTE: Crystal Violet is used because it is more stable than Gentian Violet.                              2.  Apply Solution II, (Kopeloff's Iodine modification) for 1 minute and wash off gently with distilled water.                                                                                                                                  3.  Decolorize with Solution III, Acetone Alcohol until the purple color no longer runs off the slide. (From 2 to 20 seconds depending upon thickness of specimen) Wash with distilled water.          4.  Cover slide with Solution IV, Safranin for 1 minute.  Wash with distilled water and allow to dry.

RESULTS:  Gram-positive bacteria stain purple to blue-black.                                    Gram-negative bacterial stain pink to red.

# 6098

Gram Stain Kit (Kopeloff's Modification w/Safranin
1 Kit (250 ml Ea. Sol.)

$ 102.00

CONTENTS:  Crystal Violet, Ethanol, Potassium Iodide, Iodine, Acetone, Safranin, Basic Fuchsin, Phenol  Crystal Violet, Sodium Bicarbonate, Sodium Hydroxide,  Potassium Iodide, Iodine, Ethanol, Acetone, Safranin

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

GRAM STAIN KIT
KOPELOFF'S MODIFICATION
(WITH BASIC FUCHSIN COUNTERSTAIN)

Solution IA - Crystal Violet
Solution IB - Sodium Bicarbonate, 5% (wt/vol)
Solution II  - Iodine
Solution III  - Acetone Alcohol
Solution IV - Basic Fuchsin
                                                                                                                              SUMMARY AND EXPLANATION:                                                                                        The Kopeloff's Modification of the traditional Gram Stain technique.

RECOMMENDED PROCEDURE FOR OPTIMUM RESUTLS:                                                    1.  Cover the fixed slide with Solution IA and then add 5 drops of Sodium Bicarbonate and blow on slide to mix.  Let stand for 15 seconds.  Continue staining as usual for Gram Stain.  Wash off gently with distilled water.                                                                                                NOTE: Crystal Violet is used because it is more stable than Gentian Violet.                              2.  Apply Solution II, (Kopeloff's Iodine modification) for 1 minute and wash off gently with distilled water.                                                                                                                                  3.  Decolorize with Solution III, Acetone Alcohol until the purple color no longer runs off the slide. (From 2 to 20 seconds depending upon thickness of specimen) Wash with distilled water.          4.  Cover slide with Solution IV, Basic Fuchsin for 1 minute.  Wash with distilled water and allow to dry.

RESULTS:  Gram-positive bacteria stain purple to blue-black.                                    Gram-negative bacterial stain pink to red.

# 6099

Gram Stain Kit (Kopeloff's Modification  w/Basic Fuchsin
1 Kit (250 ml Ea. Sol.)

$ 124.00

CONTENTS:  Crystal Violet, Ethanol, Potassium Iodide, Iodine, Acetone, Safranin, Basic Fuchsin, Phenol  Crystal Violet, Sodium Bicarbonate, Sodium Hydroxide,  Potassium Iodide, Iodine, Ethanol, Acetone, Basic Fuchsin

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

CYCLOSPORA STAIN KIT
(MODIFIED KINYOUN METHOD)

Solution I - Carbol Fuchsin
Solution II - Acid Alcohol (10% Sulfuric)
Solution III - Malachite Green

RECOMMENDED PROCEDURE:  Specimen may be prepared from fresh material or preserved in 10% buffered formalin (Product # 9700). Formalin-preserved Specimens: Mix stool specimen thoroughly and filter an adequate amount through cheesecloth into a centrifuge tube. Centrifuge for 2 minutes and decant supernatant. Smear 1 to 2 drops of specimen on a glass slide (either concentrate or original specimen). Heat fix on slide warmer until dry (about 5 minutes). Fresh Stool Specimens: Prepare smear utilizing the same procedure as for a formalin-fixed specimen. Fix slide in absolute methanol - 30 seconds. Flood slide with Sol. I for 1 minute. (Enhanced staining can be obtained by increasing time or applying heat). Rinse with distilled water and drain. Decolorize with Sol. II for 2 minutes. Rinse with distilled water and drain. Counterstain with Sol. III for 2 minutes. Rinse briefly with distilled water and drain. Dry on slide warmer until dry and mount.

RESULTS:  Cyclospora oocysts stain light pink to deep red measuring 8-10 microns.

# 6465

Cyclospora Stain Kit
1 Kit (250 ml Ea. Sol.)

$ 150.00

# 6466

Solution I - Carbol Fuchsin
1 Btl. ( 250 ml)

115.00

# 6467

Solution II - Acid Alcohol (10% Sulfuric)
1 Btl. (250 ml)

59.00

# 6468

Solution III - Malachite Green
1 Btl. (250 ml)

100.00

CONTENTS:  Basic Fuchsin, Ethanol, Phenol, Sulfuric Acid, Malachite Green

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

CRYPTOSPORIDIUM STAIN KIT
(MODIFIED KINYOUN METHOD)

Solution I - Carbol Fuchsin
Solution II - Acid Alcohol
Solution III - Concentrated Light Green

This technique aids in differentiating Crytosporidium oocysts from yeast cells.

RECOMMENDED PROCEDURE:   Specimen may be prepared from fresh material or preserved in 10% buffered formalin (Product # 9700).  Formalin-preserved Specimens: Mix stool suspension thoroughly and filter an adequate amount through cheesecloth into a centrifuge tube. Centrifuge for 2 minutes, decant supernatant and add saline to the remaining sediment. Centrifuge again and decant saline. Smears may be prepared from upper layer of sediment. Material should be spread on a clean glass slide and allowed to air dry. Fix fresh specimens in methanol and formalized specimens with heat. Flood smear with Sol. I for 1 - 2 minutes. Rinse gently with water. Decolorize with Sol. II and rinse again with water. Counterstain with Sol. III for 30 - 60 seconds. Rinse with water and allow to dry.  NOTE:  Decolorization and counterstaining times may vary according to thickness of specimen.

RESULTS:  Cryptosporidium oocysts - light pink to deep red; yeast cells - green to blue-purple.

# 6470

Cryptosporidium Stain Kit
1 Kit (250 ml Ea. Sol.)

$ 156.00

# 6471

Solution I - Carbol Fuchsin
1 Pack (Two 250 ml Btls.)

115.00

# 6472

Solution II - Acid Alcohol
1 Pack (Two 250 ml Btls.)

64.00

# 6473

Solution III - Concentrated Light Green
1 Pack (Two 250 ml Btls.)

100.00

CONTENTS:  Basic Fuchsin, Ethanol, Phenol, Glacial Acetic Acid, HCl, Light Green SF

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

WAYSON STAIN

Yersinia Pestis is a gram-negative bacillus with rounded ends occurring in pairs or very short chains. Bipolar staining ("safety pin" appearance) and vacuolated involutions forms are characteristic for the organism. Yersinia Pestis can be identified using a Gram Stain or Loeffler's Methylene Blue. Bipolar staining is characteristic and is best visualized with Wayson Stain.

RECOMMENDED PROCEDURE:   Prepared slides are air dried and heat fixed. Stain slides for 10 - 20 seconds.  Rinse with distilled water and blot dry.

RESULTS:  Yersinia Pestis organisms stain dark blue, leukocytes stain light blue or purple, and the background stains a light blue.

# 6475

Wayson Stain
1 Pack (Two 100 ml Btls.)

$ 196.00

# 6476

Wayson Stain
1 Btl. (100 ml)

121.00

CONTENTS:   Methylene Blue, Basic Fuchsin, Phenol, Ethanol

This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.

SPUTUM DIGESTANT

A digestant for sputum, gastric washings, etc., in preparing for acid-fast staining or culture.

RECOMMENDED PROCEDURE:   Mix equal amounts of sputum and sputum digestant. Mix well and incubate in 37oC water bath for 30 minutes with occasional shaking. With 2.5 normal HCl (Product # 9789), neutralize digested sputum to red end point. On an average, 1 - 3 ml may be needed. Flocculation will occur after 30 seconds. Centrifuge for 5 minutes then remove and discard supernatant fluid. Smears for acid-fast staining may be made directly or the diluted sediment may be used for culture. All normal flora is rendered non-viable.

# 6480 Sputum Digestant
1 Pack (Two 250 ml Btls.)

       $  171.00

# 6490 Sputum Digestant
1 Pack (Two 950 ml Btls.)

         232.00

# 6491 Sputum Digestant
1 Gallon (3,800 ml)

         410.00

CONTENTS:   Sodium Hydroxide, Potassium Alum, Methyl Red.

FOR IN VITRO DIAGNOSTIC USE ONLY.

ACID-FAST STAIN KIT
(MODIFICATION OF THE KINYOUN METHOD)

Solution I - Carbol Fuchsin                                                                                              Solution II - Acid Alcohol                                                                                                 Solution III - Loeffler’s Methylene Blue                                                                             Solution IIIA - Brilliant Green

RECOMMENDED PROCEDURE: Fix slide gently with heat. Flood slide with Sol. I and stain for 2 minutes or longer depending on thickness of smear. Rinse with water. Decolorize slide in Sol. II. Counter stain with Sol. III or Sol. IIIA for 2 minutes. Wash with water and allow to dry.

RESULTS:  Acid-fast bacilli stain red; all other bacteria stain blue with Sol. III, green with Sol. IIIA.

# 6500

Acid-Fast Stain Kit
1 Kit (250 ml Ea. Sol.)

$ 136.00

# 6501

Acid-Fast Stain Kit (Brilliant Green)
1 Kit (250 ml Ea. Sol.)

156.00

# 6505

Solution I - Carbol Fuchsin
1 Pack (Two 250 ml Btls.)

115.00

# 6506

Solution I - Carbol Fuchsin
1 Pack (Two 950 ml Btls.)

226.00

# 6507

Solution I - Carbol Fuchsin
1 Gallon (3,800 ml)

415.00

# 6510

Solution II - Acid Alcohol
1 Pack (Two 250 ml Btls.)

68.00

# 6511

Solution II - Acid Alcohol
1 Pack (Two 950 ml Btls.)

140.00

# 6512

Solution II - Acid Alcohol
1 Gallon (3,800 ml)

190.00

# 6515

Solution III - Methylene Blue
1 Pack (Two 250 ml Btls.)

98.00

# 6516

Solution III - Methylene Blue
1 Pack (Two 950 ml Btls.)

211.00

# 6517

Solution III - Methylene Blue
1 Gallon (3,800 ml)

362.00

# 6520

Solution IIIA - Brilliant Green
1 Pack (Two 250 ml Btls.)

171.00

# 6521

Solution IIIA - Brilliant Green
1 Pack (Two 950 ml Btls.)

267.00

# 6522

Solution IIIA - Brilliant Green
1 Gallon (3,800 ml)

436.00

CONTENTS: Phenol, Basic Fuchsin, Ethanol, HCl, Methylene Blue, Malachite Green

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

ACID-FAST STAIN KIT
(MODIFIED FOR NOCARDIA SPECIES)

Solution I - Carbol Fuchsin
Solution II - 1% Sulfuric Acid
Solution III - Loeffler’s Methylene Blue

The nocardiae, because of unusual long-chain fatty acids in their cell walls, can retain carbol fuchsin dye with a decolorizer of 1% sulfuric acid in water, whereas other aerobic branching bacilli cannot.

RECOMMENDED PROCEDURE: Organisms to be stained should be emulsified in a drop of distilled water on a glass slide. A known positive control and a known negative control should be stained along with the unknown strain. Allow to air dry and heat fix. Flood smear with Sol. I for 3 minutes. Wash with water. Decolorize briefly for 5 - 10 seconds with Sol. II. Counterstain with Sol. III for 30 seconds. Wash with water and allow to dry.  NOTE:   Due to the organisms weak or partial acid-fastness, do not exceed recommended decolorizing time.

RESULTS:  Partially acid-fast organisms show reddish to purple filaments; non-acid-fast organisms stain blue.

# 6530

Acid-Fast Stain Kit (Modified for Nocardia)
1 Kit (250 ml Ea. Sol.)

$ 150.00

# 6531

Solution I - Carbol Fuchsin
1 Pack ( Two 250ml Btls.)

115.00
# 6532

Solution II - Sulfuric Acid, 1%
1 Pack ( Two 250ml Btls.)

69.00
# 6533

Solution III - Loeffler's Methylene Blue
1 Pack ( Two 250ml Btls.)

98.00

CONTENTS:  Basic Fuchsin, Ethanol, Methylene Blue, Phenol, Sulfuric Acid

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

PPLO STAIN
(FOR MYCOPLASMA)
(DIENES AND WEINBERGER)

Mycoplasma, small bacteria without cell walls, are destroyed by the usual methods of smear preparation and heat fixation. On solid culture medium, they form small colonies and are best stained through direct staining of these young colonies.

RECOMMENDED PROCEDURE: On a clean, grease-free coverglass, smear a thin even film of stain and allow to dry. With a sterile scalpel, cut a block of agar from a fresh culture of the organism. Place on a clean glass slide with colonies facing upward. Apply coverglass, stain side down, gently on the agar block without rubbing. Seal with a melted mixture of paraffin and 10% petrolatum. Examine the surface of the agar for stained colonies under low-power objective.

RESULTS:  Mycoplasma colonies stain distinctly with dense blue centers and light blue peripheries.

# 6600

PPLO Stain
1 Pack (Two 100 ml Btls.)

$ 177.00

# 6601

PPLO Stain
1 Btl. (100 ml)

108.00

CONTENTS:   Methylene Blue, Azure II, Maltose, Sodium Carbonate

This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.

MODIFIED TRICHROME STAIN KIT
(FOR MICROSPORIDIA SPORES IN STOOL AND DUODENAL ASPIRATES)

Solution I - Formalin (10%)
Solution II - Methanol Fixative
Solution III - Modified Trichrome Stain
Solution IV - Acid Alcohol
Solution V - Ethanol (95%)
Solution VI - Ethanol
Solution VII - Xylene

This stain method was designed as a simple, non-invasive technique for the rapid detection of microsporidia spores in fecal material by light microscopy. The chromotrope based stain is a modification of the trichrome stain for intestinal protozoa.

RECOMMENDED PROCEDURE: Specimens for light microscopial examination should be prepared from a suspension of unconcentrated liquid stool (10 ul) in Sol. I (1:3 ratio). Smears are prepared directly and thinly spread on a glass slide covering a 45 x 25 mm area. Fix smears in Sol. II for 5 minutes. Stain smears with Sol. III for 90 minutes. Rinse slides in Sol. IV for 10 seconds. Rinse briefly in Sol. V to remove acid. Dehydrate in Sol. V for 5 minutes, Sol. VI for 10 minutes and Sol. VII for 10 minutes. Read microscopically under oil immersion lens (magnification x 1000).

RESULTS: Enterocytozoon bienuesi spores appear ovoid and refractile at approximately 1.5 by 0.90 um. The spore wall of properly stained smears should appear a bright pinkish red. Most bacteria and background debris stain a faint green.

# 6640

Modified Trichrome Stain Kit
1 Kit (100 ml Ea. Sol.)

$ 284.00

# 6644

Solution I - Formalin (10%)
1 Pack (Two 250 ml Btls.)

54.00

# 6647

Solution II - Methanol Fixative
1 Pack (Two 250 ml Btls.)

54.00

# 6650

Solution III - Modified Trichrome Stain
1 Btl. (250 ml)

188.00

# 6651

Solution III - Modified Trichrome Stain
1 Pack (Two 250 ml Btls.)

274.00

# 6652

Solution III - Modified Trichrome Stain
1 Btl. (950 ml)

439.00

# 6655

Solution IV - Acid Alcohol
1 Pack (Two 250 ml Btls.)

76.00

# 6658

Solution V - Ethanol (95%)
1 Pack (Two 250 ml Btls.)

61.00

# 6661

Solution VI - Ethanol
1 Pack (Two 250 ml Btls.)

70.00

# 6664

Solution VII - Xylene
1 Pack (Two 250 ml Btls.)

94.00

CONTENTS:   Chromotrope 2R, Fast Green FCF, Phosphotungstic Acid, Glacial Acetic Acid.

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

MODIFIED IRON HEMATOXYLIN STAIN SOLUTIONS

Solution IA - Alcoholic Hematoxylin
Solution IB - Iron Mordant Solution

A solution used in the Modified Iron Hematoxylin Stain Procedure that can incorporate a Carbol Fuchsin Step to screen for acid-fast organisms and other intestinal parasites.

RECOMMENDED PROCEDURE:  Working Solution:  Mix equal parts of Solution 1A and Solution 1B.  Recommended staining time is 8 minutes. 

RESULTS:  Background material should be stained various shades of gray-blue and the protozoa, with medium blue cytoplasm and dark blue nuclei.

# 6670

MODIFIED IRON HEMATOXYLIN            1 Pack (475 ml Each Solution)

     $ 168.00

CONTENTS:   Hematoxylin, Ethanol, Hydrochloric Acid, Ferrous Ammonium Sulfate, Ferric Ammonium Sulfate.

This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.

TRICHROME STAIN KIT
(FOR INTESTINAL PROTOZOA)
WITH PVA SOLUTION OR PVA SOLUTION AND SCHAUDINN
FIXATIVE FOR PVA FIXATIVE

Solution I - PVA Solution (5%)
Solution IA - Schaudinn Solution
Solution IB - Glacial Acetic Acid
Solution II - Iodine Alcohol
Solution III - Ethanol (70%)
Solution IV - Trichrome Stain
Solution V - Acid Alcohol (90%)
Solution VI - Ethanol (95%)
Solution VII - Carbol-Xylene
Solution VIII - Xylene

NOTE: THIS STAIN IS NOT TO BE CONFUSED WITH GOMORI’S TRICHROME STAIN USED FOR HISTOLOGY. IT IS A RECOMMENDED METHOD BY THE ASCP. "MANUAL-LABORATORY DIAGNOSIS OF INTESTINAL PARASITIC INFECTION".

A simple and quick technique for staining intestinal protozoa based on the principle of the attraction that these parasites show for the Chromotrope 2R dyes.

PVA Solution, when mixed with liquid stool specimens, (3 - 4 drops PVA with 1 - 2 drops of fecal material on a slide) provides excellent adhesion of specimen to slides, if allowed to dry several hours at 35oC or overnight at room temperature. Schaudinn fixative will properly fix and hold fresh stool specimens that have been thinly spread on a slide. (5 minutes at 50oC or 1 hour at room temperature) Smears may be left in fixative for several hours or 1 - 2 days without harm. PVA fixative (a combination of PVA Solution and Schaudinn’s fixative) will adhere as well as preserve organisms and prevent their loss during staining. One distinct advantage of PVA fixative is that it makes possible the successful staining of organisms in liquid specimens.

RECOMMENDED PROCEDURE:   STAINING TECHNIQUE WITH PVA FILMS:  PVA-fixed smears should be allowed to dry overnight at 37o degrees C prior to staining. Sol. II - 10 - 20 minutes. Sol. III - 3 - 5 minutes, then repeat. Sol. IV - 8 minutes. Sol. V - 10 - 20 seconds. Rinse with Sol. VI to remove acid. Sol. VI - 5 minutes. Sol. VII - 5 - 10 minutes. Sol. VIII - 10 minutes. Mount with coverslip.

STAINING TECHNIQUE WITH FRESH SPECIMENS:  Schaudinn’s fixative - 5 minutes at 50oC or 1 hour at room temperature. Sol. II - 1 minute. Sol. III - 1 minute, then repeat. Sol. IV - 8 minutes. Sol. V - 3 - 10 seconds. Sol. VI - rinse briefly, 2 changes. Sol. VII - 1 minute.Sol. VIII - l.- 3 minutes. Mount with coverslip.

NOTE:   CHANGE ALCOHOLS OFTEN.

RESULTS: The cytoplasm of thoroughly-fixed and well-stained cysts and trophozoites are blue-green tinged with purple. The nuclear chromatin, chromatoid bodies, ingested red cells, and bacteria stain red or purplish red. Other ingested particles, such as yeast or molds generally stain green, but variations frequently occur in the color reaction of ingested particles.Other ingested particles, such as yeasts or molds, generally stain green, but variations in color frequently occur. Background material usually stains green, and a color contrast with the protozoa results.

# 6680

Trichrome Stain Kit with PVA Solution
1 Kit (100 ml Ea. Sol.)

$ 310.00

# 6682

Trichrome Stain Kit with Schaudinn   Fixative For PVA Fixative
1 Kit (100 ml Ea. Sol.)

400.00

# 6685

Solution I - PVA Solution (5%)
1 Pack (Two 250 ml Btls.)

134.00

# 6686

Solution I - PVA Solution, 5 %
1 Gallon (3,800 ml)

350.00

# 6687 Solution IA - Schaudinn Solution              Solution 1B - Glacial Acetic Acid
1 Pack (Two 250 ml Btls.)
Sol. 1A-Two 250 ml Btls. Schaudinn Solution
Sol. 1B-Two 15 ml Btls. Glacial Acetic Acid

181.00

# 6688 Solution IA - Schaudinn Solution              Solution 1B - Glacial Acetic Acid
Sol. 1A-1 Gallon (3,800 ml) Sol. IB 450 ml.

446.00

# 6689 Solution II - Iodine Alcohol
1 Btl. (100ml.)

58.00

# 6690 Solution II - Iodine Alcohol
1 Pack (Two 250 ml Btls.)

      82.00

# 6691 Solution II - Iodine Alcohol
1 Gallon (3,800 ml)

205.00

# 6695 Solution III - Ethanol (70%)
1 Pack (Two 250 ml Btls.)

58.00

# 6696 Solution III - Ethanol (70%)
1 Gallon (3,800 ml)

195.00

# 6700 Solution IV - Trichrome Stain
1 Pack (Two 250 ml Btls.)

134.00

# 6701 Solution IV - Trichrome Stain
1 Pack (Two 950 ml Btls.)

211.00

# 6702 Solution IV - Trichrome Stain
1 Gallon (3,800 ml)

368.00

# 6705 Solution V - Acid Alcohol (90%)
1 Pack (Two 250 ml Btls.)

76.00

# 6706 Solution V - Acid Alcohol (90%)
1 Gallon (3,800 ml)

198.00

# 6710 Solution VI - Ethanol (95%)
1 Pack (Two 250 ml Btls.)

60.00

# 6711 Solution VI - Ethanol (95%)
1 Gallon (3,800 ml)

201.00

# 6715 Solution VII - Carbol-Xylene
1 Pack (Two 250 ml Btls.)

195.00

# 6716 Solution VII - Carbol-Xylene
1 Gallon (3,800 ml)

399.00

# 6720 Solution VIII - Xylene
1 Pack (Two 250 ml Btls.)

94.00

# 6721 Solution VIII - Xylene
1 Gallon (3,800 ml)

228.00

CONTENTS:  PVA Powder, Mercuric Chloride, Iodine, Ethanol, Chromotrope 2R, Light 

Green SF, Fast Green FCF, Phosphotungstic Acid, Acetic Acid (Glacial), Xylene, Pheno2

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIANOSTIC USE ONLY.

CALCOFLUOR WHITE STAIN
(FOR FUNGI)

Calcofluor white, a nonspecific fluorochrome, will bind to the cell walls of certain fungi allowing for the direct microscopic observation of fungi in wet preparations.

RECOMMENDED PROCEDURE: Add a drop of stain to specimen on clean glass slide and coverslip. Place slide face down on paper toweling and push down gently allowing excess fluid to flow out of the edges of the coverslip on to the paper towels. Examine slide under UV light.

RESULTS: Fungi elements fluoresce blue-white or green depending on filter system used i.e., a G-365 excitation filter and an LP 420 barrier filter produce light of blue-white wavelength, whereas, a K530 excitation filter and a BG 12 barrier filter produce light of green wavelength.

# 6725 Calcofluor White Stain
1 Pack (Two 100 ml Btls.)

$ 190.00

# 6726 Calcofluor White Stain
1 Btl. (100 ml)

112.00

CONTENTS:  Calcofluor White, Evans Blue.

This product is made from certified dye.
FOR IN VITRO DIAGNOSTIC USE ONLY.

LACTOPHENOL COTTON BLUE STAIN
(FOR FUNGI
)

RECOMMENDED PROCEDURE:  Mix culture material with stain (loopful) and coverslip. Lactic acid acts as a preservative for fungi, killed by phenol and stained by cotton blue. If bubbles are present, heat gently over flame.

RESULTS:  Fungal elements are stained a deep blue; background is pale blue.

# 6730 Lactophenol Cotton Blue Stain
1 Pack (Two 100 ml Btls.)

$ 190.00

# 6731 Lactophenol Cotton Blue Stain
1 Btl. (100 ml)

112.00

CONTENTS:   Phenol, Lactic Acid, Glycerol, Aniline Blue

This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.

ALBERT’S STAIN SOLUTION KIT
(FOR DIPTHERIA BACILLI)

Albert’s Stain Solution I
Albert’s Stain Solution II

This method differentiates volutin granules in the cells, and reveals the barred staining patterns of the cytoplasmic region.

RECOMMENDED PROCEDURE:  Stain heat-fixed smear with Sol. I for 3 - 5 minutes. Wash in water, blot and air dry. Stain with Sol. II for 1 minute. Wash again, blot and air dry.

RESULTSVolutin granules - dark blue or black; cytoplasm-light green; barred patterns - dark green.

# 6750 Albert’s Stain Solution Kit
1 Kit (100 ml Ea. Sol.)

$ 171.00

# 6760 Albert’s Stain Solution I
1 Pack (Two 100 ml Btls.)

171.00

# 6770 Albert’s Stain Solution II
1 Pack (Two 100 ml Btls.)

150.00

CONTENTS: Toluidine Blue, Malachite Green, Ethanol, Acetic Acid, Iodine, Potassium Iodide.

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

MIF STAIN AND FIXATIVE KIT
(MERTHIOLATE-IODINE-FORMALDEHYDE)

Solution I - MF Stock Solution
Solution II - Lugol’s Iodine

Stool preservation and staining can be accomplished simultaneously with this kit. When used as a stain, direct smear identification of intestinal protozoa and of helminth eggs may be performed immediately. As a preservative, laboratory diagnosis can be delayed without deterioration of organisms.

RECOMMENDED PROCEDURE:   Mix 2.35 ml of Sol. I with 0.15 ml of Sol. II.  This is mixed with approximately 0.25 grams of feces (about twice the size of a pea) in a well-stoppered tube. To examine, use a medicine dropper to draw off a drop of the mixture from the layer of sediment.

RESULTS: Trophozoites and cysts should stain an eosin color.

# 6780

MIF Stain and Fixative Kit
1 Kit (250 ml and 100 ml)

$ 171.00

# 6781

Sol. I - MF Stock Solution
1 Btl. (250 ml)

130.00

# 6782

Sol. II - Lugol’s Iodine
1 Btl. (100 ml)

82.00

CONTENTS: Glycerol, Formaldehyde, Tincture Merthiolate, Iodine, Potassium Iodide

FOR IN VITRO DIAGNOSTIC USE ONLY.

SPORE STAIN KIT
(WIRTZ-CONKLIN   METHOD)

(RECOMMENDED FOR STAINING BACILLUS SPECIES ANTHRACIS, CEREUS, ETC.)

Solution I - Malachite Green (5%)
Solution II - Aqueous Safranin (0.5%)

RECOMMENDED PROCEDURE:   Fix smear with heat.  Apply Solution I - Malachite Green, and heat until steam rises.  Continue for 3 to 6 minutes.  Rinse under running tap water.   Counterstain with Soluton II - Safranin for 30 seconds.  Wash very lightly with water, blot dry, and examine.

RESULTS:  Spores are seen as green spherules in red-stained rods or with attached red-stained debris.

# 6797

Spore Stain Kit (Conklin-Wirtz)
1 Kit (250 ml Ea. Sol.)

$ 228.00

# 6798

Solution I - Aqueous Malachite Green (5%)
1 Btl. (250 ml)

  138.00

# 6799

Solution II - Aqueous Safranin (0.5%)
1 Btl. (250 ml)

 110.00

CONTENTS:   Malachite Green, Safranin

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

SPORE STAIN KIT

Solution I - Basic Carbol Fuchsin
Solution II - Acetic Acid (5%)
Solution III - Loeffler’s Methylene Blue
Solution IIIA - Brilliant Green

RECOMMENDED PROCEDURE:   Heat fix smear. Apply Sol. I and heat over flame until steam rises. Continue for 5 minutes. Decolorize with Sol. II until smear assumes a light pink color. Counterstain with Sol. III or Sol. IlIA for 3 minutes. Wash with water and allow to dry.

RESULTS:  Bacteria - blue or green depending upon counter stain; spores-red.

# 6800

Spore Stain Kit (Loeffler's Methylene Blue)
1 Kit (250 ml Ea. Sol.)

$ 228.00

# 6805

Spore Stain Kit (Brilliant Green)
1 Kit (250 ml Ea. Sol.)

228.00

# 6810

Solution I - Carbol Fuchsin
1 Btl. (250 ml)

100.00

# 6820

Solution II - Acetic Acid (5%)
1 Btl. (250 ml)

82.00

# 6830

Solution IIII - Methylene Blue
1 Btl. (250 ml)

98.00

# 6840

Solution III - Brilliant Green
1 Btl. (250 ml)

98.00

CONTENTS:   Basic Fuchsin, Phenol, Ethanol, Acetic Acid, Methylene Blue, Malachite Green

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

LUGOL’S IODINE SOLUTION

Used primarily for the observation of protozoan cysts in wet mount preparations of fecal specimens.

RESULTS:   Iodine enhances the nuclear structure and stains glycogen masses dark brown.

# 6849

Lugol’s Iodine Solution
1 Btl. (100 ml)

$ 87.00

# 6850

Lugol’s Iodine Solution
1 Pack (Two 100 ml Btls.)

 184.00

# 6851

Lugol’s Iodine Solution
1 Pack (Two 250 ml Btls.)

228.00

# 6860

Lugol’s Iodine Solution
1 Pack (Two 950 ml Btls.)

308.00

# 6865

Lugol’s Iodine Solution
1 Gallon  (3,800 ml)

581.00

CONTENTS:  Iodine, Potassium Iodide

FOR IN VITRO DIAGNOSTIC USE ONLY.

CAPSULE STAIN KIT
(HISS METHOD)

Solution I - Aqueous Crystal Violet (1%)
Solution II - Aqueous Copper Sulfate (20%)

RECOMMENDED PROCEDURE:  Grow organisms in ascitic fluid or serum medium, or mix a drop of serum with specimen and from this mixture, prepare smear. Air dry. Cover smear with Sol. for 2 minutes. Wash off the stain with Sol. II. The amount of washing must be determined empirically.

RESULTS:  Capsules appear as faint blue halos around dark blue to purple cells.

# 6880

Capsule Stain Kit
1 Kit (100 ml Ea. Sol.)

$  171.00

# 6890

Capsule Stain Kit
1 Kit (250 ml Ea. Sol.)

284.00

CONTENTS:   Crystal Violet, Copper Sulfate

This solution is made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

EOSINOPHIL STAIN KIT

Solution I - Eosin Y
Solution II - Buffer Solution
Solution III - Methylene Blue

Used in staining eosinophils from secretions of the nasal passage, sinuses, bronchi and urinary sediment.

RECOMMENDED PROCEDURE:   Make thin smears and heat fix or air dry. Apply Sol. I for 30 seconds. Add Sol. II to cover slide. Mix by blowing gently on slide. Allow to stain 3 - 5 minutes. Rinse with distilled water and drain completely or blot dry. Cover slide with Sol. III and immediately rinse with water. Drain and allow to dry.

RESULTS:  Eosinophils stain with red cytoplasm and bright red granules; nucleus stains pale blue.

# 6920

Eosinophil Stain Kit
1 Kit. (250 ml ea. Sol.)

$ 236.00

# 6921

Solution I - Eosin Y
1 Btl. (250 ml)

100.00

# 6922

Solution II - Buffer Solution
1 Btl. (250 ml)

65.00

# 6923

Solution III - Methylene Blue
1 Btl. (250 ml)

125.00

CONTENTS:  Eosin Y, Methanol, Buffer Salts, Methylene Blue

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

FLUORESCENT STAIN KIT
(FOR ACID-FAST BACTERIA)

Solution I - Auramine Phenol
Solution II - Acid Alcohol
Solution III - Potassium Permanganate (0.5%)

This technique uses a phenolated fluorescent dye in place of carbol fuchsin.

RECOMMENDED PROCEDURE:  Stain heat-fixed smears 2 - 3 minutes in Sol. I. Wash in tap water. Destain 3 - 5 minutes in Sol. II. Wash in tap water. Flood smear with Sol. III      for 2 - 4 minutes. Wash in tap water. Allow to dry.

RESULTS:  Acid-fast organisms should appear fluoresced against a dark background.

# 6940

Fluorescent Stain Kit
1 Kit (250 ml Ea. Sol.)

$  137.00

# 6950

Solution I - Auramine Phenol
1 Pack (Two 250 ml Btls.)

134.00
# 6951

Solution I - Auramine Phenol
1 Gallon (3,800 ml)

503.00

# 6960

Solution II - Acid Alcohol
1 Pack (Two 250 ml Btls.)

88.00

# 6961

Solution II - Acid Alcohol
1 Gallon (3,800 ml)

263.00
# 6970

Solution III - Potassium Permanganate (0.5%)
1 Pack (Two 250 ml Btls.)

108.00

# 6971

Solution III - Potassium Permanganate (0.5%)
1 Gallon (3,800 ml)

343.00

CONTENTS:  Auramine O, Liquid Phenol, Ethanol, HCl, Potassium Permanganate

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

LEIFSON’S BACTERIAL FLAGELLA STAIN KIT

Solution I - Basic Fuchsin, (1.2%) Alcoholic
Solution II - Tannic Acid (3%)
Solution III - Sodium Chloride (1.5%)
Solution IV - Methylene Blue

Bacterial flagella are below the visual limit in size, approximately 0.1 to 0.3u in diameter. Flagella stains increase the width of these delicate appendages by forming a precipitate at the surface of the flagella making them visible with the optical microscope. The preparation may be used to establish polar or lateral distribution of flagella on a bacterial cell.

RECOMMENDED PROCEDURE:  Preparation of Slides: Slides must be clean and grease free. To assure this, soak slides for 4 days in a slide cleaning solution (Product # 6981). Thoroughly wash slides in distilled water and allow to dry.  Working Solution:  Mix equal parts of each solution (I, II, III) in a glass vessel and tightly stopper. Allow to stand 2 hours prior to use. NOTE:  Working stain solution will stain flagella satisfactorily for only 2 - 3 days; therefore, only prepare as needed. This solution precipitates rapidly at room temperature. Use only the clear supematant for staining. From a centrifuged 24 hour broth culture of a motile species of bacteria, a loopful of sediment is placed at the end of the slide. Tilt the slide allowing the liquid to run slowly to the other end and air dry. Quickly add 1 ml of stain mixture. Allow to stand 5 - 15 minutes at room temperature. When precipitate forms over the entire area of the slide, carefully wash off by flooding with water. Counterstain with 1:l0 dilution of Sol. IV for 1 minute. Wash and drain dry.

RESULTS:  Flagella stain bright red.

# 6980

Leifson’s Bacterial Flagella Stain Kit
1 Kit (250 ml Ea. Sol.)

$ 293.00

# 6981

Acid Solution
1 Pack (Two 250 ml Btls.)

132.00

CONTENTS:  Basic Fuchsin, Ethanol, Tannic Acid, Sodium Chloride, Methylene Blue, HCl

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

ZIEHL- NEELSEN STAIN KIT
(FOR ACID-FAST BACTERIA)

Solution I - Carbol Fuchsin
Solution II - Acid Alcohol
Solution III - Loeffler’s Methylene Blue

RECOMMENDED PROCEDURE:  Flood slide with Sol. I and heat until steam rises. Use low or intermittent heat to maintain steam for 3 - 5 minutes. After cooling, wash briefly with water and decolorize with Sol. II. Wash with water and counterstain with Sol. III for 20 - 30 seconds. Rinse with water, allow to dry and examine.

RESULTS:  Acid-fast organisms stain red; other organisms and material stain blue.

# 7000

Ziehl-Neelsen Stain Kit
1 Kit (250 ml Ea. Sol.)

$  240.00

# 7010

Solution I - Carbol Fuchsin
1 Pack (Two 250 ml Btls.)

185.00

# 7020

Solution I - Carbol Fuchsin
1 Pack (Two 950 ml Btls.)

293.00

# 7021 Solution I - Carbol Fuchsin
1 Gallon (3,800 ml)

    421.00

# 7030

Solution II - Acid Alcohol
1 Pack (Two 250 ml Btls.)

137.00

# 7040

Solution II - Acid Alcohol
1 Pack (Two 950 ml Btls.)

182.00

# 7041 Solution II - Acid Alcohol
1 Gallon (3,800 ml)

   201.00

# 7050

Solution III - Loeffler’s Methylene Blue
1 Pack (Two 250 ml Btls.)

150.00

# 7060

Solution III - Loeffler’s Methylene Blue
1 Pack (Two 950 ml Btls.)

236.00

# 7061 Solution III - Loeffler's Methylene Blue
1 Gallon (3,800 ml)

368.00

CONTENTS:  Basic Fuchsin, Phenol, Ethanol, HCl, Methylene Blue

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

CHLAMYDIAL STAIN KIT

Solution I - Giemsa Stain
Solution II - Buffer Solution
Solution III - Iodine Stain

RECOMMENDED PROCEDURE:  Giemsa Buffer Solution:  1 part of Sol. I to 40 or 50 parts of Sol. II. (If a darker stain is desired, increase ratio of stain to buffer.) Place fixed smear into Giemsa Buffer Sol. for 1 hour. (Time may vary according to intensity of stain desired.)  NOTE:  Giemsa Buffer Sol. should be freshly made. Slide is then rinsed rapidly in 95% ethanol to remove excess dye and to enhance differentiation.   Iodine Method:  Place fixed smear into Sol. III for 3 - 5 minutes. Slide is examined as a wet mount.  NOTE:  The slide may be decolorized with methyl alcohol and restained with Giemsa stain.

RESULTS:  Giemsa Stain:  The elementary bodies stain a reddish purple. The initial bodies are more basophilic, staining bluish as do bacteria. Iodine Stain:  The matrix of inclusions may appear as a reddish-brown mass recognizable under low power. Check with higher objective to confirm.

# 7100

Chlamydial Stain Kit
1 Kit (100 ml and 250 ml)

$  171.00

CONTENTS: Giemsa Stain, Methanol, Buffer Salts, Iodine, Potassium Iodide

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

PNEUMOCYSTIS CARINII STAIN KIT
(MODIFIED TOLUIDINE BLUE O STAIN)

Solution I - Toluidine Blue 0 Stain
Solution II - Ethanol (95%)
Solution III - Ethanol
Solution IV - Xylene

Pneumocystis carinii is an opportunist which causes a diffuse interstitial pneumonia in patients with impaired immune systems. The disease itself can be classified as epidemic or sporadic; the latter occurring in patients who have an underlying immunosuppression. In this procedure, a sulfation reagent of glacial acetic acid and sulfuric acid (not included) is used for the removal of background material. This allows the Pneumocystis carinii cysts to be visualized more easily after staining with Toluidine Blue 0. Diagnosis of Pneumocystis carinii pneumonia can frequently be made on bronchoalveolar lavage (BAL) or on touch preparations of pulmonary tissue (open lung biopsies and transbronchial biopsies).

RECOMMENDED PROCEDURES:   BAL Specimens: Using a 50 ml tube, centrifuge for 15 minutes at 2000 x g. Aspirate all but the bottom 5 ml of supernatant. With a Pasteur pipette, aspirate the sediment plus approximately 1 ml of the remaining 5 ml of fluid. Transfer material to a 15 ml centrifuge tube, gently mix and prepare smear by spreading a drop over a 1 cmm area. If concentrated specimen is very thick or mucoid, spread over entire slide with care being taken not to make the smear too thick. Dry the slides on a heating block at 50 - 55oC. Allow to cool before staining.                                     Touch Preparations:  When dry, process in same manner as slides of BAL.  Preparation of Sulfation Reagent:  In a dry coplin jar submerged in a plastic tub filled with cool water (not below 10oC), mix 45 ml of glacial acetic acid with 15 ml of concentrated sulfuric acid and stir gently with glass rod. Carefully place smears in reagent for 10 minutes. Mix immediately and again after 5 minutes. Gently rinse with water for 10 minutes. Drain excess water and stain with Sol. I for 3 - 5 minutes. Rinse with Sol. II followed by Sol. III for 10 seconds each. Clear in Sol. IV, two changes each for 10 seconds and mount.

RESULTS: The cyst forms appear as lavender structures (cup-shaped) approximately 5 urn in diameter. The cyst outline is distinct, and the internal region stains uniformly.

NOTE:   A negative bronchoalveolar lavage does not rule out an infection with Pneumocystis carinii. Other diagnostic procedures may be necessary.

# 7150

Pneumocystis Carinii Stain Kit
1 Kit (250 ml Ea. Sol.)

$ 240.00

# 7151

Solution I - Toluidine Blue O Stain
1 Pack (Two 250 ml Btls.)

190.00

# 7152

Solution II - Ethanol (95%)
1 Pack (Two 250 ml Btls.)

76.00

# 7153

Solution III - Ethanol
1 Pack (Two 250 ml Btls.)

82.00

# 7154

Solution IV - Xylene
1 Pack (Two 250 ml Btls.)

171.00

CONTENTS:   Toluidine Blue O, HCl, Ethanol, Xylene

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

GRAM-WEIGART STAIN KIT
(FOR PNEUMOCYSTIS CARINII)

Solution I - Eosin Y, (1%) Aqueous
Solution IIA - Crystal Violet
Solution IIB - Aniline Oil (2.3%) Aqueous
Solution III - Grams Iodine
Solution IV - Aniline Oil/Xylene
Solution V - Xylene

This stain kit is useful in demonstrating Pneumocystis carinii in smears of sputum, transtracheal aspirates, and bronchial washings, however, lung biopsy specimens obtained by percutaneous needle aspiration provide a better opportunity for diagnosis. Tissue sections and imprints may also be used. Prior to staining, imprints and smears must be air-dried and fixed in methanol.

RECOMMENDED PROCEDURE:   Working Solution: Mix 10 ml of Sol. IIA with 90 ml of Sol. IIB. Stain air-dried smears or imprints in Sol. I for 3 - 5 minutes. Rinse with water. Stain in working solution for 5 - 10 minutes. Rinse in Sol. III for 5 - 10 minutes. Rinse in water and blot dry. Decolorize in Sol. IV and rinse in Sol. V.

RESULTS:  Pneumocystis appears blue to deep purple against a pink background.

# 7160 Gram-Weigart Stain Kit
1 Kit (100 ml Ea. Sol.)

$ 240.00

# 7161 Solution I - Eosin Y (1%) Aqueous
1 Btl. (250 ml)

82.00

# 7162 Solution IIA - Crystal Violet
1 Btl. (250 ml)

82.00

# 7163 Solution IIB - Aniline Oil ( 2.3%)
1 Btl. (250 ml)

100.00

# 7164 Solution III - Grams Iodine
1 Btl. (250 ml)

68.00

# 7165 Solution IV - Aniline Oil/Xylene
1 Btl. (250 ml)

169.00

# 7166 Solution V - Xylene
1 Btl. (250 ml)

130.00

CONTENTS:   Eosin Y, Crystal Violet, Ethanol, Aniline Oil, Iodine, Potassium Iodide, Xylene

These solutions are made from certified dyes (when applicable.
FOR IN VITRO DIAGNOSTIC USE ONLY.

MACCHIAVELLO STAIN KIT
(FOR RICKETTSIAE AND CHLAMYDIAE)

Solution I - Basic Fuchsin
Solution II - Citric Acid (0.5%)
Solution III - Methylene Blue
Solution IIIA - Brilliant Green

Rickettsiae and chlamydiae are considered to be small bacteria since they are prokaryotic cells possessing DNA and RNA and they reproduce by the process of binary fission. They differ from all other bacteria by being obligate intracellular parasites. Since they are gram negative, gram stain does not differentiate them from the cellular material with which they are usually associated. The Macchiavello stain is considered to be one of the best differential stains available. The mechanism of this staining procedure is based on the affinity of cationic (basic) dyes for rickettsial and chlamydial organisms. This is emphasized in the decolorization step where cellular material is decolorized faster than the microorganisms.

RECOMMENDED PROCEDURE:   Prepare a smear from tissue or yolk-sac material on a clean glass slide and allow to air dry. Lightly heat fix slide. Smears should be processed soon after preparation and not allowed to remain at room temperature unstained. Cover smear with Sol. I for 5 minutes. Drain off excess dye. Wash slide in water and then rinse briefly with Sol. II. Rinse smear in water again. Counterstain with Sol. III or Sol. IIIA for 10 seconds. Rinse again, blot dry and read microscopically.  NOTE:  Exposure to citric acid for more than a few seconds will decolorize the organisms staining them blue instead of red.

RESULTS:  In a properly prepared slide, rickettsiae and chlamydiae stain red, cellular material stains blue or green.

# 7250

Macchiavello Stain Kit
1 Kit (250 ml Ea. Sol.)

$ 190.00

# 7251

Macchiavello Stain Kit
1 Kit (100 ml Ea. Sol.)

130.00

# 7252

Macchiavello Stain Kit (Brilliant Green)
1 Kit (250 ml Ea. Sol.)

190.00

# 7253

Macchiavello Stain Kit (Brilliant Green)
1 Kit (100 ml Ea. Sol.)

130.00

CONTENTS:  Basic Fuchsin, Citric Acid, Methylene Blue, Malachite Green

These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.

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