- Solution I - Giemsa Stain
- Solution II - Buffer Solution
- Solution III - Iodine Stain
CONTENTS: Giemsa Stain, Methanol, Buffer Salts, Iodine, Potassium Iodide
These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.
RECOMMENDED PROCEDURE: Giemsa Buffer Solution: 1 part of Sol. I to 40 or 50 parts of Sol. II. (If a darker stain is desired, increase ratio of stain to buffer.) Place fixed smear into Giemsa Buffer Sol. for 1 hour. (Time may vary according to intensity of stain desired.) NOTE: Giemsa Buffer Sol. should be freshly made. Slide is then rinsed rapidly in 95% ethanol to remove excess dye and to enhance differentiation. Iodine Method: Place fixed smear into Sol. III for 3 - 5 minutes. Slide is examined as a wet mount. NOTE: The slide may be decolorized with methyl alcohol and restained with Giemsa stain.
RESULTS: Giemsa Stain: The elementary bodies stain a reddish purple. The initial bodies are more basophilic, staining bluish as do bacteria. Iodine Stain: The matrix of inclusions may appear as a reddish-brown mass recognizable under low power. Check with higher objective to confirm.
Safety Data Sheet - Chlamydial Stain Kit (Solution I)
- UPC:
- 12352142
- Availability:
- Lead Time: 3-5 Days
- Type:
- Chlamydial Stain
- Includes:
- Giemsa Stain, Methanol, Buffer Salts, Iodine, Potassium Iodide
- Format:
- Kit
- Hazmat:
- No
- WeightUOM:
- LB