- Solution I - Carbol Fuchsin
- Solution II - Acid Alcohol (10% Sulfuric)
- Solution III - Malachite Green
CONTENTS: Basic Fuchsin, Ethanol, Phenol, Sulfuric Acid, Malachite Green
These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.
RECOMMENDED PROCEDURE: Specimen may be prepared from fresh material or preserved in 10% buffered formalin (Product # 9700). Formalin-preserved Specimens: Mix stool specimen thoroughly and filter an adequate amount through cheesecloth into a centrifuge tube. Centrifuge for 2 minutes and decant supernatant. Smear 1 to 2 drops of specimen on a glass slide (either concentrate or original specimen). Heat fix on slide warmer until dry (about 5 minutes). Fresh Stool Specimens: Prepare smear utilizing the same procedure as for a formalin-fixed specimen. Fix slide in absolute methanol - 30 seconds. Flood slide with Sol. I for 1 minute. (Enhanced staining can be obtained by increasing time or applying heat). Rinse with distilled water and drain. Decolorize with Sol. II for 2 minutes. Rinse with distilled water and drain. Counterstain with Sol. III for 2 minutes. Rinse briefly with distilled water and drain. Dry on slide warmer until dry and mount.
RESULTS: Cyclospora oocysts stain light pink to deep red measuring 8-10 microns.
- UPC:
- 12352142
- Availability:
- Lead Time: 3-5 Days
- Type:
- Cyclospora Stain
- Includes:
- Basic Fuchsin, Ethanol, Phenol, Sulfuric Acid, Malachite Green
- Format:
- Pack
- Hazmat:
- No
- WeightUOM:
- LB
