- Solution I - Crystal Violet
- Solution II - Grams Iodine
- Solution III - Acetone Alcohol
- Solution IV - Safranin
- Solution IV - Carbol Fuchsin
CONTENTS: Crystal Violet, Iodine, Potassium Oxalate, Ethanol, Acetone, Safranin, Basic Fuchsin, Phenol
These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.
RECOMMENDED PROCEDURE: Bring thin paraffin sections to water (6 microns or less). Flood sections with Sol. I for 1 minute. Rinse in water. Wash in Sol. II; then flood with Sol. II for 1 - 2 minutes. Decolorize with Sol. III until the purple color no longer runs off (approximately 20 - 30 seconds). Wash in water. Counterstain with Sol. IV or IVB for 30 seconds. Rinse in water. Blot dry and dehydrate rapidly in a mixture of 2 parts aniline and 1 part xylol, or ALLOW TO AIR DRY THOROUGHLY. Clear in 2 changes of xylol and mount.
RESULTS: Gram-positive bacteria, many fungi and fibrin - blue-black to violet. Gram-negative bacteria - red. Cytoplasm - pink.
Safety Data Sheet - Gram Stain for Bacteria in Tissue (Solution IVA)
- UPC:
- 12352142
- Availability:
- Lead Time: 3-5 Days
- Type:
- Gram Stain Kit (for Bacteria in Tissue)
- Includes:
- Crystal Violet, Iodine, Potassium Oxalate, Ethanol, Acetone, Safranin, Basic Fuchsin, Phenol
- Format:
- Bottle
- Hazmat:
- No
- WeightUOM:
- LB
