Gram Stain Kit (for Differential Staining of Bacteria) - Solution I - Crystal Violet (4 x 250mL)

$97.00
SKU:
6100
Brand:
ENG Scientific
Quantity:
4 x 250mL
Unit of Measure:
PK

A staining kit comprising Crystal Violet dye solution, designed for differential staining of bacteria in histological specimens, aiding in the visualization and differentiation of bacterial structures for research and diagnostic purposes.

(No reviews yet)
Current Stock:
Adding to cart… The item has been added
  • Solution I - Crystal Violet
  • Solution II - Grams Iodine
  • Solution III - Ethanol (70%)
  • Solution IIIA - Ethanol (95%)
  • Solution IIIB - Acetone-Alcohol
  • Solution IV - Safranin
  • Solution IVA - Basic Fuchsin
  • Solution IVB - Carbol Fuchsin

AVAILABLE IN VARIOUS COUNTERSTAINS AS FOLLOWS:

Safranin - Routinely used for gram negative bacteria.
Basic Fuchsin - More intense overall staining to clearly identify weak gram-negative bacteria and also aids in the detection of Bordatella.
Carbol Fuchsin - A counterstain of greater overall intensity to easily identify Legionella.

Also available with various decolorizers to suit individual preference of technique. Convenient flip-top cap for 250 ml and 950 ml sizes.

CONTENTS: Crystal Violet, Ethanol, Potassium Iodide, Iodine, Acetone, Safranin, Basic Fuchsin, Phenol

These solutions are made from certified dyes (when applicable).

FOR IN VITRO DIAGNOSTIC USE ONLY.

RECOMMENDED PROCEDURE: Cover fixed smear with Sol. I for 1 minute and rinse gently with distilled water. Apply Sol. II for 1 minute and rinse gently with distilled water. Decolorize with Sol. III, Sol. IIIA, or Sol. IIIB until the purple color no longer runs off the slide, (from 2 - 20 seconds depending upon decolorizer and thickness of smear) and rinse with distilled water. Counterstain with Sol. IV, Sol. IVA or Sol. IVB for 1 minute and rinse with distilled water. Allow to dry and examine under oil immersion.

NOTE: For a faster decolorizer, use Sol.IIIB - Acetone-Alcohol. For a more intense counterstain, we recommend Sol. IVA - Basic Fuchsin or Sol. IVB - Carbol Fuchsin.

RESULTS: Gram-positive bacteria stain purple to blue black. Gram-negative bacteria stain pink to red.

Safety Data Sheet - Gram Stain Crystal Violet (Solution I)

UPC:
12352142
Availability:
Lead Time: 3-5 Days
Type:
Gram Stain
Includes:
Crystal Violet, Ethanol, Potassium Iodide, Iodine, Acetone, Safranin, Basic Fuchsin, Phenol
Format:
Pack
Hazmat:
No
WeightUOM:
LB