- Solution I - Basic Fuchsin, (1.2%) Alcoholic
- Solution II - Tannic Acid (3%)
- Solution III - Sodium Chloride (1.5%)
- Solution IV - Methylene Blue
Bacterial flagella are below the visual limit in size, approximately 0.1 to 0.3u in diameter. Flagella stains increase the width of these delicate appendages by forming a precipitate at the surface of the flagella making them visible with the optical microscope. The preparation may be used to establish polar or lateral distribution of flagella on a bacterial cell.
CONTENTS: Basic Fuchsin, Ethanol, Tannic Acid, Sodium Chloride, Methylene Blue, HCl
These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.
RECOMMENDED PROCEDURE: Preparation of Slides: Slides must be clean and grease free. To assure this, soak slides for 4 days in a slide cleaning solution (Product # 6981). Thoroughly wash slides in distilled water and allow to dry. Working Solution: Mix equal parts of each solution (I, II, III) in a glass vessel and tightly stopper. Allow to stand 2 hours prior to use.
NOTE: Working stain solution will stain flagella satisfactorily for only 2 - 3 days; therefore, only prepare as needed. This solution precipitates rapidly at room temperature. Use only the clear supematant for staining. From a centrifuged 24 hour broth culture of a motile species of bacteria, a loopful of sediment is placed at the end of the slide. Tilt the slide allowing the liquid to run slowly to the other end and air dry. Quickly add 1 ml of stain mixture. Allow to stand 5 - 15 minutes at room temperature. When precipitate forms over the entire area of the slide, carefully wash off by flooding with water. Counterstain with 1:l0 dilution of Sol. IV for 1 minute. Wash and drain dry.
RESULTS: Flagella stain bright red.
- UPC:
- 12352142
- Availability:
- Lead Time: 3-5 Days
- Type:
- Leifson's Bacterial Flagella Stain
- Includes:
- Basic Fuchsin, Ethanol, Tannic Acid, Sodium Chloride, Methylene Blue, HCl
- Format:
- Pack
- Hazmat:
- No"ShortDescription=Specialized kit for staining Chlamydia bacteria. Includes specific staining solutions."
- WeightUOM:
- LB
