A solution that stains all white blood cells dark violet distinguishing them from red blood cells.
CONTENTS: Sodium chloride, Sodium sulfate, Sodium phosphate, Potassium phosphate, Formalin, Methyl Violet.
This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.
RECOMMENDED PROCEDURE: Draw venous or capillary blood to the 1.0 mark in a white cell pipette. Draw eosinophil solution to the 11.0 mark and mix gently for 30 seconds.
NOTE: Prolonged, vigorous shaking will tend to cause rupturing of eosinophils. Allow to stand between 5 - 15 minutes. Shake again, charge chamber and allow cells to settle before counting. 1. Prepare a 1:200 dilution of blood with Natt-Herrick's stain (add 20 ul blood to 4 ml of Natt-Herrick's stain). 2. Mix well, and leave at room temperature for 5 minutes: then fill both sides of a hemocytometer with the stained blood. 3. After 5 minutes, perform a white blood cell count, using 10X objective. That is, count all white blood cells in the 4 large corner squares on both sides of the hemocytometer chamber (the counts within each square should be within 10 percent of each other). Add all 8 counts together and use this total count to calculate.
Total # WBC's X 2000 = # WBC's/ul blood
(8)
All white blood cells will stain dark violet.
- UPC:
- 12352142
- Availability:
- Lead Time: 3-5 Days
- Type:
- Natt-Herrick's Stain
- Includes:
- Sodium chloride, Sodium sulfate, Sodium phosphate, Potassium phosphate, Formalin, Methyl Violet.
- Format:
- Pack
- Hazmat:
- No
- WeightUOM:
- LB
