- Solution IA - Methylene Blue
- Solution IB - Crystal Violet
- Solution II - Bismarck Brown
CONTENTS: Bismarck Brown, Methyl Green Methylene Blue, Crystal Violet, Bismarck Brown, Glacial Acetic Acid, Ethanol
These solutions are made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.
RECOMMENDED PROCEDURE: Specimens - intestinal (calciform cells), tracheal and embryonic tissue. Stain in Sol. I for 5 - 10 minutes. Rinse in 95% Ethanol. Transfer to Sol. II until the slide appears dark green. Dehydrate, clear and mount in balsam. Mix two parts of Solution IA - Methylene Blue with one part Solution IB - Crystal Violet. Place stain mixture onto slide for 10 - 15 seconds. Afterwards, allow the excess stain to run off the slide. Add Bismarck Brown for 45 seconds. Rinse slide with tap water. Allow slide to dry and then view.
RESULTS: Cartilage - dark brown; mucin - light brown; nuclei of all the cells - green. Examine under oil immersion at 1000X with direct illumination. Blue violet is positive (either entire cell or intracellular granules); yellow - brown is negative.
Safety Data Sheet - Methylene Blue (IA)
Safety Data Sheet - Crystal Violet (IB)
Safety Data Sheet - Bismarck Brown with Methyl Green Stain (Solution II)
- UPC:
- 12352142
- Availability:
- Lead Time: 3-5 Days
- Type:
- Neisser Stain
- Includes:
- Bismarck Brown, Methyl Green Methylene Blue, Crystal Violet, Bismarck Brown, Glacial Acetic Acid, Ethanol
- Format:
- Kit
- Hazmat:
- No
- WeightUOM:
- LB
