Mycoplasma, small bacteria without cell walls, are destroyed by the usual methods of smear preparation and heat fixation. On solid culture medium, they form small colonies and are best stained through direct staining of these young colonies.
CONTENTS: Methylene Blue, Azure II, Maltose, Sodium Carbonate
This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.
RECOMMENDED PROCEDURE: On a clean, grease-free coverglass, smear a thin even film of stain and allow to dry. With a sterile scalpel, cut a block of agar from a fresh culture of the organism. Place on a clean glass slide with colonies facing upward. Apply coverglass, stain side down, gently on the agar block without rubbing. Seal with a melted mixture of paraffin and 10% petrolatum. Examine the surface of the agar for stained colonies under low-power objective.
RESULTS: Mycoplasma colonies stain distinctly with dense blue centers and light blue peripheries.
- UPC:
- 12352142
- Availability:
- Lead Time: 3-5 Days
- Type:
- PPLO Stain
- Includes:
- Methylene Blue, Azure II, Maltose, Sodium Carbonate
- Format:
- Bottle
- Hazmat:
- No
- WeightUOM:
- LB
