- Solution I Sudan Black B
- Solution II Buffer
- Solution III 70% Ethanol
- Solution IV Harris' Alum Hematoxylin
This technique may be substituted for the benzidine-peroxidase method used for the differential diagnosis of acute granulocytic leukemia, acute lymphocytic and acute myelo-monocytic leukemia.
CONTENTS: Sudan Black B, Ethanol, Buffers, Stabilizers, Hematoxylin, Potassium Alum, Mercuric Oxide
These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.
RECOMMENDED PROCEDURE: Working Solution: Prepare as needed by mixing 60 ml of Sol. I with 40 ml of Sol. II. Filter, using suction. Fixation: Place several pieces of filter paper in the bottom of a Coplin jar with screw-top lid. Moisten with 3 - 4 drops of 37% formaldehyde. Place air-dried smears in Coplin jar and cap tightly. Fix for 10 minutes. Wash in running water for 2 minutes. Place smears in working solution for 30 - 60 minutes. Wash in Sol. III for 30 - 60 seconds to remove excess stain. Wash in tap water for 2 minutes. Counterstain in Sol. IV for 10 minutes. Wash in tap water for 5 minutes.
RESULTS: Lymphocytes and normoblasts are not stained with Sudan Black B. Normal granulocyte precursors from blast cells onward show increasing sudanophilia corresponding roughly to the granules present. Promyelocytes contain a few sudanophilic granules, while mature polymorphonuclear neutrophils contain large numbers of sudanophilic granules. Auer bodies are intensely sudanophilic.
- UPC:
- 12352142
- Availability:
- Lead Time: 3-5 Days
- Type:
- Sudan Black B Stain
- Includes:
- Sudan Black B, Ethanol, Buffers, Stabilizers, Hematoxylin, Potassium Alum, Mercuric Oxide
- Format:
- Pack
- Hazmat:
- No
- WeightUOM:
- LB
