Gill-Hematoxylin Stain (Modified) - Solution I (2 x 950mL)

• Solution I - Gill-Hematoxylin, 0.2% (Modified), 0.4% or 0.6%
• Solution II - Scott’s Tap Water Substitute
• Solution III - Ethanol (95%)

An excellent nuclear stain employing a half-oxidized hematoxylin solution. (Gill, 1974) Gill Hematoxylin is a high contrast stain and a progressive hematoxylin which enchances the use of counterstains. To achieve desired stain characteristics in different specimens, the stain is available in 3 concentrations at 0.2% w/v, (Modified), 0.4% w/v, and 0.6% w/v. The modified solution at 0.2% may be preferred in cytology, however, by increasing the time (10 minutes) it can be used in histology. Gill-Hematoxylin at 0.4% is recommended for histology, however, a shorter stain time renders this solution suitable for cytologic use. For comparable results to those obtained with Harris’ Hematoxylin, Gill-Hematoxylin at 0.6% is recommended. An essential part of this stain sequence is the use of a blueing agent. We recommend Scott’s Tap Water Substitute which ensures a controlled pH and aids in cell retention on slides.

CONTENTS: Ethylene Glycol, Hematoxylin, Sodium Iodate, Aluminum Sulfate, Citric Acid, Magnesium Sulfate, Sodium Bicarbonate, Ethanol

These solutions are made from certified dyes (when applicable).

FOR IN VITRO DIAGNOSTIC USE ONLY.

RECOMMENDED PROCEDURE: Gill-Hematoxylin (Modified): Fix in Sol. III for 15 minutes. Water - 2 x (10 dips each). Gill-Hematoxylin (Modified) - 2 minutes. Water - 2 x (10 dips each). Sol. II - 1 minute. Water - 2 x (10 dips each). Sol. III - 2 x (10 dips each). Continue with preferred staining method. Gill-Hematoxylin 0.4 % or 0.6 %: Xylene - 3 x (2 minutes each). Absolute Ethanol - 3 x (30 seconds each). Water - 2 x (10 dips each). Gill Hematoxylin 0.4% or 0.6% - 3 minutes. Water - 2 x (10 dips each). Sol. II - 2 minutes. Water - 2 x (10 dips each). Continue with preferred staining method.

RESULTS: Nuclear chromatin stains blue to blue-black; cytoplasm faintly tinted.

Safety Data Sheet - Gill-Hematoxylin Stain Kit, Modified (Solution I)