- Solution I - Eosin Y, (1%) Aqueous
- Solution IIA - Crystal Violet
- Solution IIB - Aniline Oil (2.3%) Aqueous
- Solution III - Grams Iodine
- Solution IV - Aniline Oil/Xylene
- Solution V - Xylene
This stain kit is useful in demonstrating Pneumocystis carinii in smears of sputum, transtracheal aspirates, and bronchial washings, however, lung biopsy specimens obtained by percutaneous needle aspiration provide a better opportunity for diagnosis. Tissue sections and imprints may also be used. Prior to staining, imprints and smears must be air-dried and fixed in methanol.
CONTENTS: Eosin Y, Crystal Violet, Ethanol, Aniline Oil, Iodine, Potassium Iodide, Xylene
These solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.
RECOMMENDED PROCEDURE: Working Solution: Mix 10 ml of Sol. IIA with 90 ml of Sol. IIB. Stain air-dried smears or imprints in Sol. I for 3 - 5 minutes. Rinse with water. Stain in working solution for 5 - 10 minutes. Rinse in Sol. III for 5 - 10 minutes. Rinse in water and blot dry. Decolorize in Sol. IV and rinse in Sol. V.
RESULTS: Pneumocystis appears blue to deep purple against a pink background.
Safety Data Sheet - Gram-Weigart Stain Kit (Solution I)
Safety Data Sheet - Gram-Weigart Stain Kit (Solution IIA)
Safety Data Sheet - Gram-Weigart Stain Kit (Solution IIB)
Safety Data Sheet - Gram-Weigart Stain Kit (Solution III)
- UPC:
- 12352142
- Availability:
- Lead Time: 3-5 Days
- Type:
- Gram Stain
- Includes:
- Eosin Y, Crystal Violet, Ethanol, Aniline Oil, Iodine, Potassium Iodide, Xylene
- Format:
- Kit
- Hazmat:
- No
- WeightUOM:
- LB
