Gram-Weigart Stain - Solution V - Xylene (250mL)

$165.00
SKU:
7166
Brand:
ENG Scientific
Quantity:
250mL
Unit of Measure:
BO

Modified hematoxylin stain for nuclear and cytoplasmic staining. Ensures superior contrast.

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  • Solution I - Eosin Y, (1%) Aqueous
  • Solution IIA - Crystal Violet
  • Solution IIB - Aniline Oil (2.3%) Aqueous
  • Solution III - Grams Iodine
  • Solution IV - Aniline Oil/Xylene
  • Solution V - Xylene

This stain kit is useful in demonstrating Pneumocystis carinii in smears of sputum, transtracheal aspirates, and bronchial washings, however, lung biopsy specimens obtained by percutaneous needle aspiration provide a better opportunity for diagnosis. Tissue sections and imprints may also be used. Prior to staining, imprints and smears must be air-dried and fixed in methanol.

CONTENTS: Eosin Y, Crystal Violet, Ethanol, Aniline Oil, Iodine, Potassium Iodide, Xylene

These solutions are made from certified dyes (when applicable).

FOR IN VITRO DIAGNOSTIC USE ONLY.

RECOMMENDED PROCEDURE: Working Solution: Mix 10 ml of Sol. IIA with 90 ml of Sol. IIB. Stain air-dried smears or imprints in Sol. I for 3 - 5 minutes. Rinse with water. Stain in working solution for 5 - 10 minutes. Rinse in Sol. III for 5 - 10 minutes. Rinse in water and blot dry. Decolorize in Sol. IV and rinse in Sol. V.

RESULTS: Pneumocystis appears blue to deep purple against a pink background.

Safety Data Sheet - Gram-Weigart Stain Kit (Solution V)

UPC:
12352142
Availability:
Lead Time: 3-5 Days
Type:
Gram Stain
Includes:
Eosin Y, Crystal Violet, Ethanol, Aniline Oil, Iodine, Potassium Iodide, Xylene
Format:
Bottle
Hazmat:
No
WeightUOM:
LB