H-PACK II (Wright Giemsa Stain) (1 Pack)

These stain packs are designed to eliminate problems with precipitate, artifacts, drying of slides, and overstaining which causes abnormal staining characteristics of all cellular elements, i.e., false toxic granulation. One adjustment should be all that is necessary to obtain desired color and intensity. Buffer solution compliments individual stain lots to allow consistent staining characteristics. Normal and abnormal WBC’s and platelets are vividly demonstrated. Bacteria within leukocytes can be clearly observed. RBC staining gives excellent results of normal cellular elements including all abnormalities and inclusion bodies. Parasites, such as malaria, are well-defined. Bone marrow preps can be stained routinely. Freezing or high temperature will not cause change in staining ability. Stain pack is designed to be used at room temperature. SHAKE WELL BEFORE.

USE PRECAUTIONS: The solvent for the stain is methanol, which is flammable. Store at room temperature. IN THE EVENT OF COLD WEATHER, allow solution to return to room temperature and SHAKE WELL BEFORE USE.

CONTENTS: Wright Stain, Giemsa Stain, May-Grunwald Stain, Methanol, Buffers, Stabilizers

These solutions are made from certified dyes (when applicable).

FOR IN VITRO DIAGNOSTIC USE ONLY.

RECOMMENDED USES: H-PACK & H-PACK II may be used on all specimens requiring a WBC differential, particularly spinal fluid and nasal smears, and any procedure requiring a Wright counterstain. Other uses include LE preps, parasitic elements such as malaria, thicker buffy coat smears, mature and immature elements of routine bone marrow smears, inclusion bodies such as basophilic stippling, etc. All stain clearly and distinctly.

RECOMMENDED PROCEDURE: Prior to inserting stain pack, all cannulas, tubing and platen surfaces should be cleaned with methanol. For optimum results, new tubing should be soaked in methanol. In order to prevent staining inconsistencies or artifacts due to quality of slides or humid conditions affecting the smear, we recommend fixing in methanol. (After fixing, it is not necessary to dry slide prior to insertion into machine.) Crenation of red blood cells can be caused by mechanical changes in preparation of slide prior to staining. H-PACK: Place stain pack into your instrument with the cut out holes facing forward. Insert cannulas through plastic bottles until flush. NOTE: If cannulas are dull and do not puncture bottles smoothly, use an 18-20 gauge needle to puncture bottles prior to inserting cannulas. H-PACK II: The cannulas are inserted into the appropriate containers of solution. The stain container has two cannulas, one inserted at each end of the container. The buffer and rinse containers have one cannula each.

RECOMMENDATION FOR MACHINE SETTINGS: Due to the inconsistency in the pump motors of the Ames HEMA-TEK Slide Stainers and the constant wear of the pump tubing, we recommend that a daily setting be made in order to obtain consistent results from day to day.

H-PACK WRIGHT, WRIGHT GIEMSA AND MAY-GRUNWALD GIEMSA STAINS: Ames Hema-Tek and Ames Hema-Tek 1000: All pumps should initially be set on maximum by rotating the pumps completely to the right. For a more acid (red) stain, keep buffer and rinse pumps on full and reduce stain pumps by rotating setting to the left. For a more alkaline (blue) stain, keep stain and rinse pumps on full and reduce buffer by rotating setting to the left. Ames Hema-Tek 2000: All pumps should initially be set to +2. For a more acid (red) stain, adjust only stain pump by rotating setting to 0 or -2. For a more alkaline (blue) stain, adjust only buffer pump by rotating setting to 0 or -2. Due to the unstable characteristics of formulating a stain pack that contains both a Wright, Giemsa or May-Grunwald stains, the presence of some residue on the platen will be seen after use. It is suggested that the platen surface be cleaned at frequent intervals to prevent build-up of stain residue on the platen.

H-PACK II WRIGHT GIEMSA STAIN: Since the H-PACK II stain requires a shorter staining time, both quantities of solution and time may be decreased. Setting the rinse down to the minimum, stain volume and stain intensity control may be decreased yielding excellent staining quality.

NOTE: RINSE SHOULD BE SET ON FULL AT ALL TIMES TO AID IN CLEANING AND DRYING OF SLIDES.

TROUBLESHOOTING COMMON STAINING PROBLEMS: Ames HEMA-TEK Slide Stainers: Change all tubing monthly and flush stain line with methanol. Use 25 gauge needle and syringe and place in stain opening on platen. Remove stain pump tubing and check to determine if methanol is flushing out from rear connector. Clean platen and surrounding wells. Remove all debris and be careful not to bend finger switches. Make sure finger switches are clean and floating freely. Run blank slide through stainer and check pump times. Stain pump should be activated when center of slide is over opening on platen and pump should run for about 8 seconds. Be sure the slide is completely covered with the stain. Buffer pump should be activated when edge of slide is over opening on platen and pump should run for about 45 seconds. Rinse pump should be activated when edge of slide is over opening on platen. This should run for 40 - 45 seconds and pump should remain on full. Adjust finger switches to correct.

NOTE: Due to the variations in the oxidation of a methylene blue stain, our Wright, Wright Giemsa and May-Grunwald Giemsa stain products are offered with a matched buffer solution of specific pH in order to obtain consistent and uniform staining characteristics from lot to lot. As a result, we recommend our matched buffer solution be used for obtaining optimum results. However, stain and buffer are available separately.

Safety Data Sheet (Buffer) - H-PACK II (Wright Giemsa Stain)

Safety Data Sheet (Rinse) - H-PACK II (Wright Giemsa Stain)

Safety Data Sheet (Stain) - H-PACK II (Wright Giemsa Stain)