Acid-Fast Stain Kit (Nocardia Species) - Solution III - Loeffner's Methylene Blue (2 x 250mL)

$127.00
SKU:
6533
Brand:
ENG Scientific
Quantity:
2 x 250mL
Unit of Measure:
PK

Loeffner's Methylene Blue solution III for Acid-Fast staining of Nocardia species, supplied in two bottles for counterstaining acid-fast bacteria, ensuring contrast in stained samples.

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  • Solution I Carbol Fuchsin
  • Solution II Sulfuric Acid, 1%
  • Solution III Loeffner's Methylene Blue

The nocardiae, because of unusual long-chain fatty acids in their cell walls, can retain carbol fuchsin dye with a decolorizer of 1% sulfuric acid in water, whereas other aerobic branching bacilli cannot.

CONTENTS: Basic Fuchsin, Ethanol, Methylene Blue, Phenol, Sulfuric Acid

These solutions are made from certified dyes (when applicable).

FOR IN VITRO DIAGNOSTIC USE ONLY.

RECOMMENDED PROCEDURE: Organisms to be stained should be emulsified in a drop of distilled water on a glass slide. A known positive control and a known negative control should be stained along with the unknown strain. Allow to air dry and heat fix. Flood smear with Sol. I for 3 minutes. Wash with water. Decolorize briefly for 5 - 10 seconds with Sol. II. Counterstain with Sol. III for 30 seconds. Wash with water and allow to dry. NOTE: Due to the organisms weak or partial acid-fastness, do not exceed recommended decolorizing time.

RESULTS: Partially acid-fast organisms show reddish to purple filaments; non-acid-fast organisms stain blue

Safety Data Sheet - Acid-Fast Stain Kit (Solution III) Modified for Nocardia

UPC:
12352142
Availability:
Lead Time: 3-5 Days
Type:
Acid-Fast Stain
Includes:
Basic Fuchsin, Ethanol, Methylene Blue, Phenol, Sulfuric Acid
Format:
Pack
Hazmat:
No
WeightUOM:
LB